Detailed information of TA system TA212454

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 2962423..2962835
• Replicon: chromosome
• Accession: NZ_CP081007
• Organism: Escherichia coli strain XL1-Blue

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: K3826_RS14060
• Protein ID: WP_000132601.1
• Coordinates: 2962494..2962835 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	2962423..2962499 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3826_RS14050 (2959286)	2959286..2960875	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
K3826_RS14055 (2960872)	2960872..2962266	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
- (2962423)	2962423..2962499	-	77	NuclAT_15	-	Antitoxin
- (2962423)	2962423..2962499	-	77	NuclAT_15	-	Antitoxin
- (2962423)	2962423..2962499	-	77	NuclAT_15	-	Antitoxin
- (2962423)	2962423..2962499	-	77	NuclAT_15	-	Antitoxin
- (2962423)	2962423..2962499	-	77	NuclAT_16	-	Antitoxin
- (2962423)	2962423..2962499	-	77	NuclAT_16	-	Antitoxin
- (2962423)	2962423..2962499	-	77	NuclAT_16	-	Antitoxin
- (2962423)	2962423..2962499	-	77	NuclAT_16	-	Antitoxin
K3826_RS14060 (2962494)	2962494..2962835	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
K3826_RS14065 (2962997)	2962997..2964376	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
K3826_RS14070 (2964376)	2964376..2965422	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T212454 WP_000132601.1 NZ_CP081007:2962494-2962835 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T212454 NZ_CP107038:c1252278-1252024 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT212454 NZ_CP081007:c2962499-2962423 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin