Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1363329..1364128 Replicon chromosome
Accession NZ_CP081007
Organism Escherichia coli strain XL1-Blue

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag K3826_RS06365 Protein ID WP_000347273.1
Coordinates 1363664..1364128 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K3826_RS06360 Protein ID WP_001307405.1
Coordinates 1363329..1363664 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3826_RS06345 (1359114) 1359114..1359884 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
K3826_RS06350 (1359900) 1359900..1361234 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K3826_RS06355 (1361609) 1361609..1363180 + 1572 WP_001273747.1 galactarate dehydratase -
K3826_RS06360 (1363329) 1363329..1363664 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K3826_RS06365 (1363664) 1363664..1364128 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K3826_RS06370 (1364183) 1364183..1364992 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K3826_RS06375 (1365241) 1365241..1366521 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
K3826_RS06380 (1366544) 1366544..1367017 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
K3826_RS06385 (1367028) 1367028..1367399 + 372 Protein_1260 PTS sugar transporter subunit IIC -
K3826_RS06390 (1367395) 1367395..1367952 + 558 Protein_1261 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T212441 WP_000347273.1 NZ_CP081007:1363664-1364128 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T212441 NZ_CP107020:c2709863-2709606 [Brachybacterium huguangmaarense]
GTGCTCCTCGTCTGGAGCTCGAGCGCCTGGGACGACTACCTCTGGTGGCAGTCGCAGGATCGCAAGACCCTGCGGCGGAT
CAACACGCTCATCCAGGACATCTCCCGCAACGGCCACGACGGCATCGGCAAGCCCGAGGCCCTGAAGCATGACTTCGCGG
GCTATTGGTCCCGCCGCATCACCGACGAGCACCGCCTCGTCTACAAGATCGAAGGCGACGAGGTGCGCATCGCGGCGTGC
CGGTACCACTACCGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT212441 WP_001307405.1 NZ_CP081007:1363329-1363664 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT212441 NZ_CP107020:c2710131-2709865 [Brachybacterium huguangmaarense]
ATGAAGACGCTCAGCTACACGGAATCTCGCGCACGGTACGCCGAGGTCCTGGACGGCGTCGTCGCGGACCGTGAGGAAGT
GGTCATCACGCGCGCCGGGCACGAGCCCGTCGTCATCGTGTCCCTCGACGACTACGAGTCCCTGCGCGAGACGGCGTATC
TGATGCGGTCGCCCGCCAACGCCCGCCGCCTGCTGGATGCCATGGAGCAGCTCGAGGGCGGCCGCGGGGCGCGTCACGAG
CTCGTCGACGTCGATGGCGACGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References