Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1320281..1321008 Replicon chromosome
Accession NZ_CP081007
Organism Escherichia coli strain XL1-Blue

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag K3826_RS06140 Protein ID WP_000550189.1
Coordinates 1320694..1321008 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K3826_RS06135 Protein ID WP_000560266.1
Coordinates 1320281..1320697 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3826_RS06125 (1315441) 1315441..1317792 + 2352 WP_000695487.1 alpha-glucosidase -
K3826_RS06130 (1318218) 1318218..1320236 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
K3826_RS06135 (1320281) 1320281..1320697 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
K3826_RS06140 (1320694) 1320694..1321008 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
K3826_RS06145 (1321292) 1321292..1322428 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
K3826_RS06150 (1322513) 1322513..1323016 + 504 WP_001333820.1 M48 family metallopeptidase -
K3826_RS06155 (1323093) 1323093..1323785 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
K3826_RS06160 (1323864) 1323864..1324850 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T212440 WP_000550189.1 NZ_CP081007:c1321008-1320694 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T212440 NZ_CP107020:c923625-923371 [Brachybacterium huguangmaarense]
GTGCGTCTGGTGTGGGACCGCTCCGCGTGGGAGGACTACACCTCCTGGCAGACGTCCGACCGCAAGATCCTCAAGCGGAT
CAACACTCTCCTCGACGCGTGTCTGCGTGAGCCGACGACGGGCCTCGGCAAGCCCGAACCTCTCAAGTACGGGGCCCAGG
GAGCCTGGTCGCGGCGCATCACCGAGGAGCACCACCTCGTGTATCTCGTGGACGGCGACGATCTGGTGATCCTCCAGGCT
CGATACCACTACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT212440 WP_000560266.1 NZ_CP081007:c1320697-1320281 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT212440 NZ_CP107020:c923877-923626 [Brachybacterium huguangmaarense]
ATGTCCATCAGTGCGAGTGAAGCGCGCAAGACCCTGTTCCCGCTCATCGAGCGCGTCAACGACGATCGGGATGCCGTGGA
GATCGTCTCCCGCAAGGGCAACGCGGTCCTGATGTCGGCCGACGAGTACGCGGCGTGGCAGGAGACCGCCTATCTGTTCC
GATCGCCGCGGAACGCCCGGAGGCTGCTCGACGCGTACGAGCGCGCTCGCGACGGCCGGACCGAGGTCCACGAGCTCGAT
CGGGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References