Detailed information of TA system TA212387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3536791..3537545
• Replicon: chromosome
• Accession: NZ_CP080960
• Organism: Salmonella enterica subsp. enterica serovar Typhi strain ISP2825

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q8Z2U0
• Locus tag: K3U68_RS17395
• Protein ID: WP_000558160.1
• Coordinates: 3536791..3537102 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K3U68_RS17400
• Protein ID: WP_001259009.1
• Coordinates: 3537099..3537545 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U68_RS17365 (K3U68_17365)	3532457..3533359	+	903	WP_000331359.1	formate dehydrogenase subunit beta	-
K3U68_RS17370 (K3U68_17370)	3533356..3533991	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
K3U68_RS17375 (K3U68_17375)	3533988..3534917	+	930	WP_000027727.1	formate dehydrogenase accessory protein FdhE	-
K3U68_RS17380 (K3U68_17380)	3534955..3535329	-	375	WP_000238494.1	type II toxin-antitoxin system VapC family toxin	-
K3U68_RS17385 (K3U68_17385)	3535329..3535571	-	243	WP_001523745.1	CopG family transcriptional regulator	-
K3U68_RS17390 (K3U68_17390)	3535776..3536705	+	930	WP_001162859.1	alpha/beta hydrolase	-
K3U68_RS17395 (K3U68_17395)	3536791..3537102	+	312	WP_000558160.1	type II toxin-antitoxin system HigB family toxin	Toxin
K3U68_RS17400 (K3U68_17400)	3537099..3537545	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
K3U68_RS17405 (K3U68_17405)	3537560..3538501	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
K3U68_RS17410 (K3U68_17410)	3538546..3538983	-	438	WP_000560975.1	D-aminoacyl-tRNA deacylase	-
K3U68_RS17415 (K3U68_17415)	3538980..3539852	-	873	WP_000921423.1	virulence factor BrkB family protein	-
K3U68_RS17420 (K3U68_17420)	3539846..3540445	-	600	WP_000965695.1	glucose-1-phosphatase	-
K3U68_RS17425 (K3U68_17425)	3540636..3541439	-	804	WP_000059689.1	DeoR family transcriptional regulator	-
K3U68_RS17430 (K3U68_17430)	3541473..3542369	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12449.38 Da
• Isoelectric Point: 8.4020

>T212387 WP_000558160.1 NZ_CP080960:3536791-3537102 [Salmonella enterica subsp. enterica serovar Typhi]
VHVISRKLFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNEE

Nucleotide

• Length: 312 bp

>T212387 NZ_CP107005:1967178-1967281 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT212387 WP_001259009.1 NZ_CP080960:3537099-3537545 [Salmonella enterica subsp. enterica serovar Typhi]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT212387 NZ_CP107005:c1967319-1967048 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A714FUZ5