Detailed information of TA system TA212362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2405881..2406443
• Replicon: chromosome
• Accession: NZ_CP080958
• Organism: Citrobacter amalonaticus strain C3H

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6Z3F2
• Locus tag: K1713_RS11535
• Protein ID: WP_044257939.1
• Coordinates: 2405881..2406159 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K1713_RS11540
• Protein ID: WP_043000693.1
• Coordinates: 2406159..2406443 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1713_RS11515 (K1713_11515)	2401006..2401437	-	432	WP_043000697.1	peroxiredoxin OsmC	-
K1713_RS11520 (K1713_11520)	2401665..2401808	+	144	WP_042319017.1	stationary-phase-induced ribosome-associated protein	-
K1713_RS11525 (K1713_11525)	2401980..2403677	+	1698	WP_044257943.1	malate dehydrogenase	-
K1713_RS11530 (K1713_11530)	2403986..2405701	+	1716	WP_044257940.1	ABC-F family ATP-binding cassette domain-containing protein	-
K1713_RS11535 (K1713_11535)	2405881..2406159	+	279	WP_044257939.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K1713_RS11540 (K1713_11540)	2406159..2406443	+	285	WP_043000693.1	HigA family addiction module antitoxin	Antitoxin
K1713_RS11545 (K1713_11545)	2406488..2407090	-	603	WP_044257938.1	inorganic diphosphatase	-
K1713_RS11550 (K1713_11550)	2407217..2407873	-	657	WP_043000691.1	formate dehydrogenase-N subunit gamma	-
K1713_RS11555 (K1713_11555)	2407866..2408750	-	885	WP_043000690.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10525.03 Da
• Isoelectric Point: 8.1206

>T212362 WP_044257939.1 NZ_CP080958:2405881-2406159 [Citrobacter amalonaticus]
MIMNFRHKGLRDLFLHGKTSGVLAKHVKRLRHRLAVIDAAGCVNDINMPGYRLHPLSGDRDGVWSISVSGNWRMTFEFVN
GDAYILDDEDYH

Nucleotide

• Length: 279 bp

>T212362 NZ_CP107004:c1812379-1812005 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10370.07 Da
• Isoelectric Point: 10.2982

>AT212362 WP_043000693.1 NZ_CP080958:2406159-2406443 [Citrobacter amalonaticus]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAQAQ
KSVDVSRLRRLAVQ

Nucleotide

• Length: 285 bp

>AT212362 NZ_CP107004:c1812836-1812468 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A7L6Z3F2