Detailed information of TA system TA212360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 40484..41151
• Replicon: plasmid pRoPD630_2
• Accession: NZ_CP080956
• Organism: Rhodococcus opacus PD630

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A3G9M8M4
• Locus tag: K2Z90_RS40725
• Protein ID: WP_005249444.1
• Coordinates: 40484..40840 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: K2Z90_RS40730
• Protein ID: WP_143767564.1
• Coordinates: 40837..41151 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2Z90_RS40690 (K2Z90_008088)	35674..36087	+	414	WP_005249436.1	YkvA family protein	-
K2Z90_RS40695 (K2Z90_008089)	36281..36883	+	603	WP_005249437.1	hypothetical protein	-
K2Z90_RS40700 (K2Z90_008090)	37212..37478	-	267	WP_025433707.1	hypothetical protein	-
K2Z90_RS40705 (K2Z90_008091)	37656..38624	+	969	WP_005249440.1	DNA ligase	-
K2Z90_RS40710 (K2Z90_008092)	38891..39238	-	348	WP_005249441.1	Lsr2 family protein	-
K2Z90_RS40715 (K2Z90_008093)	39405..39776	+	372	WP_025433708.1	hypothetical protein	-
K2Z90_RS40720 (K2Z90_008094)	39818..40246	-	429	WP_005249443.1	hypothetical protein	-
K2Z90_RS40725 (K2Z90_008095)	40484..40840	+	357	WP_005249444.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K2Z90_RS40730 (K2Z90_008096)	40837..41151	+	315	WP_143767564.1	XRE family transcriptional regulator	Antitoxin
K2Z90_RS40735	41202..41534	-	333	WP_226955877.1	hypothetical protein	-
K2Z90_RS40740 (K2Z90_008098)	42000..42449	-	450	WP_005249447.1	hypothetical protein	-
K2Z90_RS40745 (K2Z90_008099)	42494..43255	-	762	WP_005249448.1	hypothetical protein	-
K2Z90_RS41860	43905..44027	+	123	WP_264297301.1	hypothetical protein	-
K2Z90_RS40750 (K2Z90_008101)	44307..44756	+	450	WP_005249449.1	DUF6325 family protein	-
K2Z90_RS40755 (K2Z90_008102)	44777..45028	+	252	WP_025433710.1	SHOCT domain-containing protein	-
K2Z90_RS40760 (K2Z90_008103)	45060..45449	+	390	WP_005249450.1	SHOCT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..167985	167985

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (35-95)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13206.11 Da
• Isoelectric Point: 6.4784

>T212360 WP_005249444.1 NZ_CP080956:40484-40840 [Rhodococcus opacus PD630]
VGKWTVEVGLVEEWLLALDQSSYEQVVAALELLAERGPQLGRPLVDTVKASRHKNIKELRPGSSGRSELRVLFAFDPERK
AILLVAGNKAGQWTTWYKVNIPIADDRFAAHLDELEGR

Nucleotide

• Length: 357 bp

>T212360 NZ_CP107004:1800235-1800486 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11829.47 Da
• Isoelectric Point: 5.7791

>AT212360 WP_143767564.1 NZ_CP080956:40837-41151 [Rhodococcus opacus PD630]
VNTMAKSLEQLLAERPVDRGAVEAHKARMIDEVRAYRLQELRESMAMTQKQLAERLGVGQNRVSNIEHGDLERVQIDTLR
RYVEAVGGALRVEVELGDERFQIA

Nucleotide

• Length: 315 bp

>AT212360 NZ_CP107004:1800483-1800737 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A3G9M8M4