Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1340661..1341436 Replicon chromosome
Accession NZ_CP080761
Organism Diaphorobacter sp. MNS-0

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7T1GPJ5
Locus tag K2L43_RS06405 Protein ID WP_011804662.1
Coordinates 1340978..1341436 (+) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A7T1DU90
Locus tag K2L43_RS06400 Protein ID WP_038204260.1
Coordinates 1340661..1340978 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K2L43_RS06385 (K2L43_06385) 1336988..1338082 + 1095 WP_009518528.1 signal peptidase II -
K2L43_RS06395 (K2L43_06395) 1338479..1340362 - 1884 WP_009518527.1 MobH family relaxase -
K2L43_RS06400 (K2L43_06400) 1340661..1340978 + 318 WP_038204260.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
K2L43_RS06405 (K2L43_06405) 1340978..1341436 + 459 WP_011804662.1 type II toxin-antitoxin system YhaV family toxin Toxin
K2L43_RS06410 (K2L43_06410) 1341463..1341840 + 378 WP_011804663.1 DUF3742 family protein -
K2L43_RS06415 (K2L43_06415) 1341856..1343373 - 1518 WP_011804664.1 conjugal transfer protein TraG N-terminal domain-containing protein -
K2L43_RS06420 (K2L43_06420) 1343388..1343747 - 360 WP_009518522.1 hypothetical protein -
K2L43_RS06425 (K2L43_06425) 1343744..1345138 - 1395 WP_220638720.1 integrating conjugative element protein -
K2L43_RS06430 (K2L43_06430) 1345148..1346095 - 948 WP_121382470.1 TIGR03756 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 1304449..1379142 74693


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-101)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17681.07 Da        Isoelectric Point: 10.3103

>T212344 WP_011804662.1 NZ_CP080761:1340978-1341436 [Diaphorobacter sp. MNS-0]
MQRHGWTLLFHDCVIEPLQKLQAAARRAQENDPKGFESNANVKLFRALSQLMLEVVPGDPARDEYRQGNTLGSAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

>T212344 NZ_CP107003:2671044-2671151 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11129.57 Da        Isoelectric Point: 4.3682

>AT212344 WP_038204260.1 NZ_CP080761:1340661-1340978 [Diaphorobacter sp. MNS-0]
MPAIHEVATLTSKGQVTLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNVQGLPED
LARAMLEHAGHGVNLDEEIDGEVEL

Download         Length: 318 bp

>AT212344 NZ_CP107003:c2670997-2670931 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1GPJ5

Antitoxin

Source ID Structure
AlphaFold DB A0A7T1DU90

References