Detailed information of TA system TA021232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 26788..27306
• Replicon: plasmid pSSP1
• Accession: NC_007351
• Organism: Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: SSP_RS12415
• Protein ID: WP_011304050.1
• Coordinates: 26788..27057 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: SSP_RS12420
• Protein ID: WP_011304051.1
• Coordinates: 27058..27306 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SSP_RS12395	22985..24250	+	1266	WP_011304047.1	replication initiator protein A	-
SSP_RS12400	24216..24893	+	678	WP_103314606.1	transposase	-
SSP_RS12405	24911..25189	+	279	WP_002508705.1	hypothetical protein	-
SSP_RS12410	25891..26565	+	675	WP_011304049.1	IS6 family transposase	-
SSP_RS12415	26788..27057	-	270	WP_011304050.1	Txe/YoeB family addiction module toxin	Toxin
SSP_RS12420	27058..27306	-	249	WP_011304051.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SSP_RS12425	27614..28612	-	999	WP_041785008.1	ThiF family adenylyltransferase	-
SSP_RS12430	28612..29379	-	768	WP_011304054.1	thiazole synthase	-
SSP_RS12435	29381..29581	-	201	WP_011304055.1	sulfur carrier protein ThiS	-
SSP_RS12440	29571..30683	-	1113	WP_011304056.1	FAD-binding oxidoreductase	-
SSP_RS12445	30676..31260	-	585	WP_041785010.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38454	38454
	-	flank	IS/Tn	-	-	25891..26565	674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10361.96 Da
• Isoelectric Point: 10.1510

>T21232 WP_011304050.1 NC_007351:c27057-26788 [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC]
MAKLNITFSPQAFEDYEYFQLKDRPMVKRINKLLKSINRDGALGGIGKPEKLVGSLSGYYSRRINQEHRLVYTVNDTSIM
VASCRYHYR

Nucleotide

• Length: 270 bp

>T21232 NC_007351:c27057-26788 [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC]
ATGGCTAAATTAAATATTACTTTTTCACCTCAAGCATTTGAAGATTATGAATACTTTCAGTTAAAAGATAGACCTATGGT
AAAGCGTATCAATAAATTGCTTAAAAGTATTAACAGAGATGGGGCTTTAGGAGGTATTGGGAAACCTGAAAAATTGGTTG
GTAGTCTTTCGGGATATTATAGTCGTCGGATTAATCAGGAACATAGATTAGTATATACGGTGAATGATACTTCAATCATG
GTTGCTTCATGTAGGTATCATTATAGATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9483.53 Da
• Isoelectric Point: 4.3525

>AT21232 WP_011304051.1 NC_007351:c27306-27058 [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC]
VTVKNYTFVRDNFRDMINKVNDDSDTITITTKDRNAVLMSEDDYDAIMETLYLQQNPSNAKHLAESIENLERGNVKSKEI
EI

Nucleotide

• Length: 249 bp

>AT21232 NC_007351:c27306-27058 [Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305 = NCTC]
GTGACGGTTAAAAATTATACGTTCGTAAGAGATAATTTTAGAGATATGATTAATAAGGTAAATGATGATAGCGATACAAT
TACGATTACAACAAAAGACCGTAACGCTGTGTTGATGTCAGAAGATGATTACGACGCAATTATGGAAACATTATATCTTC
AACAAAATCCTTCCAACGCAAAACATTTAGCAGAATCAATTGAGAATTTAGAACGTGGTAATGTTAAAAGTAAGGAAATA
GAAATATAA