212291

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4218723..4219225 Replicon chromosome
Accession NZ_CP080620
Organism Escherichia coli strain TOP10

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag K3F56_RS20210 Protein ID WP_000767829.1
Coordinates 4218723..4218977 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag K3F56_RS20215 Protein ID WP_001259255.1
Coordinates 4218974..4219225 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3F56_RS20180 (4213738) 4213738..4213965 - 228 WP_046613325.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
K3F56_RS20185 (4213979) 4213979..4215037 - 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
K3F56_RS20190 (4215216) 4215216..4216574 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
K3F56_RS20195 (4216564) 4216564..4216626 - 63 WP_010723108.1 membrane protein YoeI -
K3F56_RS20200 (4216841) 4216841..4217770 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
K3F56_RS20205 (4217816) 4217816..4218640 - 825 WP_000754737.1 SDR family oxidoreductase -
K3F56_RS20210 (4218723) 4218723..4218977 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
K3F56_RS20215 (4218974) 4218974..4219225 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
K3F56_RS20220 (4219508) 4219508..4219558 + 51 WP_001364200.1 his operon leader peptide -
K3F56_RS20225 (4219704) 4219704..4220603 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
K3F56_RS20230 (4220609) 4220609..4221913 + 1305 WP_000009594.1 histidinol dehydrogenase -
K3F56_RS20235 (4221910) 4221910..4222980 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
K3F56_RS20240 (4222980) 4222980..4224047 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T212291 WP_000767829.1 NZ_CP080620:c4218977-4218723 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T212291 NZ_CP107001:1967178-1967281 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT212291 WP_001259255.1 NZ_CP080620:c4219225-4218974 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT212291 NZ_CP107001:c1967319-1967048 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References