Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 3646193..3646886 Replicon chromosome
Accession NZ_CP080620
Organism Escherichia coli strain TOP10

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag K3F56_RS17280 Protein ID WP_000415584.1
Coordinates 3646193..3646489 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag K3F56_RS17285 Protein ID WP_000650107.1
Coordinates 3646491..3646886 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3F56_RS17245 (3641281) 3641281..3641595 - 315 WP_000958598.1 putative quinol monooxygenase -
K3F56_RS17250 (3641626) 3641626..3642207 - 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
K3F56_RS17255 (3642526) 3642526..3642858 + 333 WP_000917684.1 DUF2645 family protein -
K3F56_RS17260 (3642904) 3642904..3644253 - 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
K3F56_RS17265 (3644250) 3644250..3644909 - 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB -
K3F56_RS17270 (3645061) 3645061..3645453 + 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
K3F56_RS17275 (3645506) 3645506..3645988 + 483 WP_000183505.1 GyrI-like domain-containing protein -
K3F56_RS17280 (3646193) 3646193..3646489 + 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
K3F56_RS17285 (3646491) 3646491..3646886 + 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
K3F56_RS17290 (3647019) 3647019..3648626 + 1608 WP_001295629.1 ABC transporter substrate-binding protein -
K3F56_RS17295 (3648764) 3648764..3651022 + 2259 WP_001281881.1 DNA topoisomerase IV subunit A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T212288 WP_000415584.1 NZ_CP080620:3646193-3646489 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T212288 NZ_CP107001:1800235-1800486 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT212288 WP_000650107.1 NZ_CP080620:3646491-3646886 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT212288 NZ_CP107001:1800483-1800737 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References