Detailed information of TA system TA212283

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3236570..3236982
• Replicon: chromosome
• Accession: NZ_CP080620
• Organism: Escherichia coli strain TOP10

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: K3F56_RS15410
• Protein ID: WP_000132601.1
• Coordinates: 3236641..3236982 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3236570..3236646 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3F56_RS15400 (3233433)	3233433..3235022	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
K3F56_RS15405 (3235019)	3235019..3236413	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
- (3236570)	3236570..3236646	-	77	NuclAT_14	-	Antitoxin
- (3236570)	3236570..3236646	-	77	NuclAT_14	-	Antitoxin
- (3236570)	3236570..3236646	-	77	NuclAT_14	-	Antitoxin
- (3236570)	3236570..3236646	-	77	NuclAT_14	-	Antitoxin
- (3236570)	3236570..3236646	-	77	NuclAT_15	-	Antitoxin
- (3236570)	3236570..3236646	-	77	NuclAT_15	-	Antitoxin
- (3236570)	3236570..3236646	-	77	NuclAT_15	-	Antitoxin
- (3236570)	3236570..3236646	-	77	NuclAT_15	-	Antitoxin
K3F56_RS15410 (3236641)	3236641..3236982	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
K3F56_RS15415 (3237144)	3237144..3238523	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
K3F56_RS15420 (3238523)	3238523..3239569	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T212283 WP_000132601.1 NZ_CP080620:3236641-3236982 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T212283 NZ_CP107001:714669-714965 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT212283 NZ_CP080620:c3236646-3236570 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin