Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 959486..960285 Replicon chromosome
Accession NZ_CP080620
Organism Escherichia coli strain TOP10

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag K3F56_RS04480 Protein ID WP_000347273.1
Coordinates 959821..960285 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K3F56_RS04475 Protein ID WP_001307405.1
Coordinates 959486..959821 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3F56_RS04460 (955271) 955271..956041 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
K3F56_RS04465 (956057) 956057..957391 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
K3F56_RS04470 (957766) 957766..959337 + 1572 WP_001273747.1 galactarate dehydratase -
K3F56_RS04475 (959486) 959486..959821 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K3F56_RS04480 (959821) 959821..960285 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K3F56_RS04485 (960340) 960340..961149 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K3F56_RS04490 (961398) 961398..962678 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
K3F56_RS04495 (962701) 962701..963174 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
K3F56_RS04500 (963185) 963185..963556 + 372 Protein_892 PTS sugar transporter subunit IIC -
K3F56_RS04505 (963552) 963552..964109 + 558 Protein_893 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 950338..971269 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T212268 WP_000347273.1 NZ_CP080620:959821-960285 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T212268 NZ_CP107000:2247036-2247323 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT212268 WP_001307405.1 NZ_CP080620:959486-959821 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT212268 NZ_CP107000:2246797-2247036 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References