Detailed information of TA system TA212263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5642856..5643463
• Replicon: chromosome
• Accession: NZ_CP080597
• Organism: Pseudomonas sp. ERGC3:05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: K2E96_RS26275
• Protein ID: WP_221294181.1
• Coordinates: 5643149..5643463 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A172YY36
• Locus tag: K2E96_RS26270
• Protein ID: WP_064451284.1
• Coordinates: 5642856..5643149 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2E96_RS26230 (K2E96_25835)	5638451..5638663	+	213	WP_064451291.1	cysteine-rich CWC family protein	-
K2E96_RS26235 (K2E96_25840)	5638663..5639355	+	693	WP_064451290.1	pseudouridine synthase	-
K2E96_RS26240 (K2E96_25845)	5639660..5640544	+	885	WP_064451289.1	alpha/beta hydrolase	-
K2E96_RS26250 (K2E96_25855)	5641028..5641551	+	524	Protein_5167	DUF3833 domain-containing protein	-
K2E96_RS26255 (K2E96_25860)	5641575..5641880	+	306	WP_221294183.1	DUF6482 family protein	-
K2E96_RS26260 (K2E96_25865)	5641961..5642200	-	240	WP_064451286.1	DUF6124 family protein	-
K2E96_RS26265 (K2E96_25870)	5642633..5642773	+	141	Protein_5170	transcriptional regulator	-
K2E96_RS26270 (K2E96_25875)	5642856..5643149	-	294	WP_064451284.1	helix-turn-helix domain-containing protein	Antitoxin
K2E96_RS26275 (K2E96_25880)	5643149..5643463	-	315	WP_221294181.1	hypothetical protein	Toxin
K2E96_RS26280 (K2E96_25885)	5643652..5644846	-	1195	Protein_5173	MFS transporter	-
K2E96_RS26285 (K2E96_25890)	5644843..5646006	-	1164	WP_221294179.1	MFS transporter	-
K2E96_RS26290	5646102..5646323	+	222	Protein_5175	LysR family transcriptional regulator	-
K2E96_RS26295 (K2E96_25895)	5646327..5646997	+	671	Protein_5176	LysR family substrate-binding domain-containing protein	-
K2E96_RS26300 (K2E96_25900)	5647165..5647347	+	183	WP_221294177.1	type II toxin-antitoxin system HicA family toxin	-
K2E96_RS26305 (K2E96_25905)	5647370..5647782	+	413	Protein_5178	type II toxin-antitoxin system HicB family antitoxin	-
K2E96_RS26310 (K2E96_25910)	5647898..5648398	+	501	WP_221294176.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-83)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11932.73 Da
• Isoelectric Point: 6.9798

>T212263 WP_221294181.1 NZ_CP080597:c5643463-5643149 [Pseudomonas sp. ERGC3:05]
VIFVESPVFTEDLPALLSEESYGEFQEFLAENPTAGDVIQGTNGLRKIRWALQGRGKRGGVRVIYYHLCMAYQIRLILIY
RKGIKDDLSAVEKSVLSEINRRWE

Nucleotide

• Length: 315 bp

>T212263 NZ_CP107000:c1123275-1122202 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10986.60 Da
• Isoelectric Point: 9.8595

>AT212263 WP_064451284.1 NZ_CP080597:c5643149-5642856 [Pseudomonas sp. ERGC3:05]
MEKKLFNRLVESMTQMDEIARGERAPSREFFVDALKVKEIRQATGLSQARFAKAIDVPVGTLQNWEQGRREPEGPAKALL
RALYNDPDHVLAALGHR

Nucleotide

• Length: 294 bp

>AT212263 NZ_CP107000:c1122209-1121838 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A172YY36