Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1836373..1837043 Replicon chromosome
Accession NZ_CP080591
Organism Cronobacter sakazakii strain Crono-589

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag K1721_RS08670 Protein ID WP_032989869.1
Coordinates 1836373..1836669 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K1721_RS08675 Protein ID WP_032989868.1
Coordinates 1836702..1837043 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K1721_RS08650 (K1721_08655) 1831987..1832814 - 828 WP_004387342.1 ammonia-dependent NAD(+) synthetase -
K1721_RS08655 (K1721_08660) 1833057..1833398 + 342 WP_004387341.1 osmotically-inducible lipoprotein OsmE -
K1721_RS08660 (K1721_08665) 1833512..1835767 - 2256 WP_032989870.1 catalase HPII -
K1721_RS08665 (K1721_08670) 1835964..1836209 + 246 WP_255212326.1 cell division activator CedA -
K1721_RS08670 (K1721_08675) 1836373..1836669 + 297 WP_032989869.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K1721_RS08675 (K1721_08680) 1836702..1837043 + 342 WP_032989868.1 HigA family addiction module antitoxin Antitoxin
K1721_RS08680 (K1721_08685) 1837153..1838544 - 1392 WP_004386567.1 L-cystine transporter -
K1721_RS08685 (K1721_08690) 1838680..1839270 - 591 WP_007792738.1 metal-dependent hydrolase -
K1721_RS08690 (K1721_08695) 1839384..1840139 - 756 WP_032989866.1 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase KduD -
K1721_RS08695 (K1721_08700) 1840327..1840995 - 669 WP_032989864.1 hexitol phosphatase HxpB -
K1721_RS08700 (K1721_08705) 1841154..1841690 + 537 WP_004386563.1 YniB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11390.92 Da        Isoelectric Point: 8.8587

>T212224 WP_032989869.1 NZ_CP080591:1836373-1836669 [Cronobacter sakazakii]
MQKHIGSFCDAWLAAFFLYSTPHRNIPAEIHTTLARKLDIINAATSYRDLRSPPGNRFEALSGKLQGYSSIRVNHHYRLI
FRWVEGKAEDLYLDPHDY

Download         Length: 297 bp

>T212224 NZ_CP106998:2671044-2671151 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12795.80 Da        Isoelectric Point: 8.0052

>AT212224 WP_032989868.1 NZ_CP080591:1836702-1837043 [Cronobacter sakazakii]
MKQATRKPTTPGEILVYEYLEPLGLKINEVAEMLCVHRNSVSALVNNNRKLTTDMAFRLAKAFGTSVDFWLNLQAAVDLW
EVENDMRIQEAFSRIVPAAEFIARRDAQKKAVA

Download         Length: 342 bp

>AT212224 NZ_CP106998:c2670997-2670931 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References