Detailed information of TA system TA212218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4759740..4760329
• Replicon: chromosome
• Accession: NZ_CP080589
• Organism: Xanthomonas oryzae pv. oryzicola strain GX01

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A0C5V8F2
• Locus tag: K2I41_RS21925
• Protein ID: WP_024712137.1
• Coordinates: 4759740..4760021 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K2I41_RS21930
• Protein ID: WP_011260746.1
• Coordinates: 4760039..4760329 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2I41_RS21920 (K2I41_21825)	4757384..4759645	+	2262	WP_024712136.1	exodeoxyribonuclease V subunit alpha	-
K2I41_RS21925 (K2I41_21830)	4759740..4760021	+	282	WP_024712137.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K2I41_RS21930 (K2I41_21835)	4760039..4760329	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
K2I41_RS21935 (K2I41_21840)	4761267..4762661	+	1395	WP_149621925.1	type III secretion system effector XopQ	-
K2I41_RS21940	4762764..4762951	-	188	Protein_4112	hypothetical protein	-
K2I41_RS21945 (K2I41_21845)	4763112..4763444	-	333	WP_011260743.1	hypothetical protein	-
K2I41_RS21950 (K2I41_21850)	4763699..4764004	+	306	WP_224229802.1	hypothetical protein	-
K2I41_RS21955 (K2I41_21855)	4764154..4764576	+	423	WP_044751863.1	PAAR domain-containing protein	-
K2I41_RS21960 (K2I41_21860)	4764579..4765055	+	477	WP_024712141.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11125.91 Da
• Isoelectric Point: 10.5318

>T212218 WP_024712137.1 NZ_CP080589:4759740-4760021 [Xanthomonas oryzae pv. oryzicola]
MIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYNIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T212218 NZ_CP106998:c1812379-1812005 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT212218 WP_011260746.1 NZ_CP080589:4760039-4760329 [Xanthomonas oryzae pv. oryzicola]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT212218 NZ_CP106998:c1812836-1812468 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0C5V8F2