Detailed information of TA system TA212018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 926648..927236
• Replicon: chromosome
• Accession: NZ_CP080536
• Organism: Acidithiobacillus ferriphilus strain GT2

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: K1Y48_RS04580
• Protein ID: WP_229066265.1
• Coordinates: 926648..926929 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K1Y48_RS04585
• Protein ID: WP_215849504.1
• Coordinates: 926940..927236 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1Y48_RS04560 (K1Y48_04580)	923404..924480	+	1077	WP_215849673.1	DUF4263 domain-containing protein	-
K1Y48_RS04565 (K1Y48_04585)	924552..925166	+	615	WP_215849507.1	hypothetical protein	-
K1Y48_RS04570 (K1Y48_04590)	925276..925653	+	378	WP_229066264.1	DUF2513 domain-containing protein	-
K1Y48_RS04575 (K1Y48_04595)	925728..926444	+	717	WP_215849505.1	hypothetical protein	-
K1Y48_RS04580 (K1Y48_04600)	926648..926929	+	282	WP_229066265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K1Y48_RS04585 (K1Y48_04605)	926940..927236	+	297	WP_215849504.1	HigA family addiction module antitoxin	Antitoxin
K1Y48_RS04590 (K1Y48_04610)	927934..928188	-	255	WP_215849503.1	Txe/YoeB family addiction module toxin	-
K1Y48_RS04595 (K1Y48_04615)	928185..928436	-	252	WP_035191769.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
K1Y48_RS04600 (K1Y48_04620)	928803..930314	+	1512	WP_215849502.1	PQQ-binding-like beta-propeller repeat protein	-
K1Y48_RS04605 (K1Y48_04625)	930431..931264	+	834	WP_229066266.1	ferritin-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	923404..927303	3899

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10740.16 Da
• Isoelectric Point: 7.3598

>T212018 WP_229066265.1 NZ_CP080536:926648-926929 [Acidithiobacillus ferriphilus]
MIQTFANTETEGFFVTGKSRRLPSEILKRAAMRLNQLHAATELNDLHLPPSNHLEALSGNRIGQWSIRINQQWRVCFKFE
NGDAFNVEIVDYH

Nucleotide

• Length: 282 bp

>T212018 NZ_CP106912:329340-329708 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11001.80 Da
• Isoelectric Point: 8.5298

>AT212018 WP_215849504.1 NZ_CP080536:926940-927236 [Acidithiobacillus ferriphilus]
MIYNGLTPIHPGEYLAELLEELHISQALFARTIGVSPMRISHIVKGKRPVTAELALLFAKAFEQTPQYWLNLQASYDLKT
AEKSIANRLFSIHSLAHA

Nucleotide

• Length: 297 bp

>AT212018 NZ_CP106912:329122-329343 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA