Detailed information of TA system TA211983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5332540..5333135
• Replicon: chromosome
• Accession: NZ_CP080518
• Organism: Pseudomonas aeruginosa strain YY322

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KMW38_RS24725
• Protein ID: WP_003113526.1
• Coordinates: 5332857..5333135 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KMW38_RS24720
• Protein ID: WP_003113527.1
• Coordinates: 5332540..5332845 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KMW38_RS24685 (KMW38_24685)	5327679..5328527	+	849	WP_003162406.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
KMW38_RS24695 (KMW38_24695)	5328694..5329635	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
KMW38_RS24700 (KMW38_24700)	5329752..5330366	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
KMW38_RS24705 (KMW38_24705)	5330408..5330992	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
KMW38_RS24710 (KMW38_24710)	5331033..5332133	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
KMW38_RS24720 (KMW38_24720)	5332540..5332845	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KMW38_RS24725 (KMW38_24725)	5332857..5333135	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KMW38_RS29630	5333188..5333316	-	129	Protein_4893	integrase	-
KMW38_RS24730 (KMW38_24730)	5333464..5335692	+	2229	WP_003146080.1	TonB-dependent receptor	-
KMW38_RS24735 (KMW38_24735)	5335762..5336409	-	648	WP_003095021.1	carbonate dehydratase	-
KMW38_RS24740 (KMW38_24740)	5336471..5337709	-	1239	WP_003162396.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T211983 WP_003113526.1 NZ_CP080518:c5333135-5332857 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T211983 NZ_CP106890:c5378752-5378369 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT211983 WP_003113527.1 NZ_CP080518:c5332845-5332540 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT211983 NZ_CP106890:c5378994-5378752 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: V6ALY3