Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1216215..1216942 Replicon chromosome
Accession NZ_CP080470
Organism Enterobacter hormaechei strain ECC59

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag K0823_RS05805 Protein ID WP_022650547.1
Coordinates 1216631..1216942 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag K0823_RS05800 Protein ID WP_022650546.1
Coordinates 1216215..1216634 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0823_RS05785 (K0823_05785) 1213287..1214408 - 1122 WP_023324277.1 YbdK family carboxylate-amine ligase -
K0823_RS05790 (K0823_05790) 1214514..1215404 + 891 WP_126525253.1 oxidoreductase -
K0823_RS05795 (K0823_05795) 1215457..1215891 - 435 WP_045342299.1 hypothetical protein -
K0823_RS05800 (K0823_05800) 1216215..1216634 - 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
K0823_RS05805 (K0823_05805) 1216631..1216942 - 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
K0823_RS25340 1217398..1218054 + 657 WP_022650548.1 hypothetical protein -
K0823_RS05815 (K0823_05815) 1218167..1218622 - 456 WP_220153636.1 hypothetical protein -
K0823_RS05820 (K0823_05820) 1218905..1219786 - 882 WP_022650550.1 LysR family transcriptional regulator -
K0823_RS05825 (K0823_05825) 1219892..1220671 + 780 WP_026094386.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T211927 WP_022650547.1 NZ_CP080470:c1216942-1216631 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T211927 NZ_CP106852:1951600-1951881 [Edwardsiella ictaluri]
ATGACCTATAAACTGAGCTTTGAAAAGCGAGCCCTGAAAGAGTGGAAAAAACTGGCACCGCCGATTCAAAGCCAGTTGAA
AAAGAAACTGATCGAACGCCTGGAAAACCCACATGTTCCAGCAGCACGCTTAAGTGGGCGCGCTAACCGTTATAAAATAA
AATTACGCTCTTCCGGTTACCGGTTAGTTTATGAAGTCAACGATAGCGAAATCATCCTGCTGGTGATCGCTATCGGTAAA
CGGGCCGATAACGAGGTGTACCAAGCGGCAGACAGTCGTTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT211927 WP_022650546.1 NZ_CP080470:c1216634-1216215 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT211927 NZ_CP106852:1951359-1951610 [Edwardsiella ictaluri]
ATGGCATACCAAATTCTCACGACCACCGCCGCCAGCATTACCGACCTGAAACGCAATCCAATGGGTACCATTGCTGAAGG
TGACGGCAATGCCGTAGCAATCTTAAACCGGAATGAGCCGGCCTTTTACTGCGTTCCACCGGAACTCTACGCTTACTATC
TGGAGCTGGCTGAAGATGCGGCGCTTAATCGTATCGCCGATGAGCGTCTGGAGGACGCTGAGTTTGTCAGCGTAAATATC
GATGACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References