Detailed information of TA system TA211861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5353178..5353773
• Replicon: chromosome
• Accession: NZ_CP080405
• Organism: Pseudomonas aeruginosa strain PES_P749

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: K0T28_RS24770
• Protein ID: WP_003113526.1
• Coordinates: 5353495..5353773 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K0T28_RS24765
• Protein ID: WP_003113527.1
• Coordinates: 5353178..5353483 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0T28_RS24730 (K0T28_24725)	5348395..5348646	-	252	WP_003115979.1	hypothetical protein	-
K0T28_RS24735 (K0T28_24730)	5348768..5349202	-	435	WP_009875803.1	hypothetical protein	-
K0T28_RS24740 (K0T28_24735)	5349718..5350008	-	291	WP_012614380.1	DUF5447 family protein	-
K0T28_RS24745 (K0T28_24740)	5350184..5350456	-	273	WP_004352675.1	hypothetical protein	-
K0T28_RS24750	5350568..5350840	+	273	WP_033994353.1	hypothetical protein	-
K0T28_RS24755 (K0T28_24745)	5350961..5351773	+	813	WP_079385434.1	FRG domain-containing protein	-
K0T28_RS24760 (K0T28_24750)	5351774..5352886	+	1113	WP_079385433.1	DUF262 domain-containing protein	-
K0T28_RS24765 (K0T28_24755)	5353178..5353483	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
K0T28_RS24770 (K0T28_24760)	5353495..5353773	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K0T28_RS24775	5353826..5353954	-	129	Protein_4905	integrase	-
K0T28_RS24780 (K0T28_24765)	5354102..5356330	+	2229	WP_031654229.1	TonB-dependent receptor	-
K0T28_RS24785 (K0T28_24770)	5356400..5357047	-	648	WP_003095021.1	carbonate dehydratase	-
K0T28_RS24790 (K0T28_24775)	5357109..5358347	-	1239	WP_023119842.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T211861 WP_003113526.1 NZ_CP080405:c5353773-5353495 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T211861 NZ_CP106805:c1642961-1642779 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT211861 WP_003113527.1 NZ_CP080405:c5353483-5353178 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT211861 NZ_CP106805:c1642677-1642276 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Structures

Toxin

• AlphaFold DB: V6ALY3