Detailed information of TA system TA211820

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35192..35431
• Replicon: plasmid pPK5086-97kb
• Accession: NZ_CP080372
• Organism: Escherichia coli strain PK5086

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: K0H55_RS23530
• Protein ID: WP_023144756.1
• Coordinates: 35192..35326 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	35371..35431 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0H55_RS23500 (30893)	30893..31750	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
K0H55_RS23505 (31743)	31743..31817	-	75	WP_031943482.1	RepA leader peptide Tap	-
K0H55_RS23515 (32179)	32179..33720	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
K0H55_RS23520 (33735)	33735..34481	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
K0H55_RS23525 (34641)	34641..34895	-	255	WP_000083850.1	replication regulatory protein RepA	-
K0H55_RS23530 (35192)	35192..35326	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (35371)	35371..35431	+	61	NuclAT_0	-	Antitoxin
- (35371)	35371..35431	+	61	NuclAT_0	-	Antitoxin
- (35371)	35371..35431	+	61	NuclAT_0	-	Antitoxin
- (35371)	35371..35431	+	61	NuclAT_0	-	Antitoxin
K0H55_RS23535 (35398)	35398..35684	-	287	Protein_40	DUF2726 domain-containing protein	-
K0H55_RS23540 (36197)	36197..36409	-	213	WP_013023861.1	hypothetical protein	-
K0H55_RS23545 (36540)	36540..37100	-	561	WP_000139341.1	fertility inhibition protein FinO	-
K0H55_RS23550 (37155)	37155..37901	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
K0H55_RS23555 (37913)	37913..39454	-	1542	Protein_44	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(B) / sul1 / qacE / aadA5 / aac(3)-IId	-	1..97614	97614

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T211820 WP_023144756.1 NZ_CP080372:c35326-35192 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T211820 NZ_CP106784:c4622046-4621291 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGCGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGCCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT211820 NZ_CP080372:35371-35431 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin