Detailed information of TA system TA211818

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60262..60531
• Replicon: plasmid pPK5086-tetX
• Accession: NZ_CP080371
• Organism: Escherichia coli strain PK5086

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K0H55_RS23110
• Protein ID: WP_001372321.1
• Coordinates: 60406..60531 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	60262..60327 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0H55_RS24620 (55290)	55290..55556	+	267	WP_072667006.1	hypothetical protein	-
K0H55_RS23075 (55797)	55797..56003	+	207	WP_000275858.1	hypothetical protein	-
K0H55_RS23080 (56029)	56029..56568	+	540	WP_001825195.1	single-stranded DNA-binding protein	-
K0H55_RS23085 (56631)	56631..56864	+	234	WP_000005990.1	DUF905 family protein	-
K0H55_RS23090 (56930)	56930..58888	+	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
K0H55_RS23095 (58943)	58943..59377	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K0H55_RS23100 (59374)	59374..60136	+	763	Protein_69	plasmid SOS inhibition protein A	-
K0H55_RS23105 (60105)	60105..60293	-	189	WP_001299721.1	hypothetical protein	-
- (60262)	60262..60327	+	66	NuclAT_1	-	-
- (60262)	60262..60327	-	66	NuclAT_0	-	Antitoxin
- (60262)	60262..60327	-	66	NuclAT_0	-	Antitoxin
- (60105)	60105..60329	+	225	NuclAT_0	-	-
- (60105)	60105..60329	+	225	NuclAT_0	-	-
- (60105)	60105..60329	+	225	NuclAT_0	-	-
- (60105)	60105..60329	+	225	NuclAT_0	-	-
- (60105)	60105..60329	-	225	NuclAT_0	-	-
K0H55_RS24625 (60315)	60315..60464	+	150	Protein_71	plasmid maintenance protein Mok	-
K0H55_RS23110 (60406)	60406..60531	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K0H55_RS24630 (60751)	60751..60981	+	231	WP_001426396.1	hypothetical protein	-
K0H55_RS24635 (60979)	60979..61152	-	174	Protein_74	hypothetical protein	-
K0H55_RS24640 (61222)	61222..61428	+	207	WP_000547968.1	hypothetical protein	-
K0H55_RS23115 (61453)	61453..61740	+	288	WP_000107544.1	hypothetical protein	-
K0H55_RS23120 (61861)	61861..62682	+	822	WP_001234485.1	DUF932 domain-containing protein	-
K0H55_RS23125 (62979)	62979..63626	-	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
K0H55_RS23130 (63903)	63903..64286	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K0H55_RS23135 (64477)	64477..65163	+	687	WP_000332484.1	PAS domain-containing protein	-
K0H55_RS23140 (65257)	65257..65484	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T211818 WP_001372321.1 NZ_CP080371:60406-60531 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211818 NZ_CP106784:2758432-2759007 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT211818 NZ_CP080371:c60327-60262 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin