Detailed information of TA system TA211802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1111554..1112281
• Replicon: chromosome
• Accession: NZ_CP080370
• Organism: Escherichia coli strain PK5086

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: K0H55_RS05435
• Protein ID: WP_000547564.1
• Coordinates: 1111554..1111865 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K0H55_RS05440
• Protein ID: WP_000126294.1
• Coordinates: 1111862..1112281 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0H55_RS05405 (1106696)	1106696..1108405	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
K0H55_RS05410 (1108415)	1108415..1108957	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
K0H55_RS05415 (1108957)	1108957..1109724	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
K0H55_RS05420 (1109721)	1109721..1110131	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
K0H55_RS05425 (1110124)	1110124..1110594	+	471	WP_060621146.1	hydrogenase maturation peptidase HycI	-
K0H55_RS05430 (1110619)	1110619..1111380	+	762	WP_001026446.1	hypothetical protein	-
K0H55_RS05435 (1111554)	1111554..1111865	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
K0H55_RS05440 (1111862)	1111862..1112281	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
K0H55_RS05445 (1112395)	1112395..1113819	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
K0H55_RS05450 (1113828)	1113828..1115285	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
K0H55_RS05455 (1115545)	1115545..1116555	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
K0H55_RS05460 (1116704)	1116704..1117231	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T211802 WP_000547564.1 NZ_CP080370:1111554-1111865 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T211802 NZ_CP106775:c950889-950470 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT211802 WP_000126294.1 NZ_CP080370:1111862-1112281 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT211802 NZ_CP106775:c951125-950889 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5