Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 656169..656968 Replicon chromosome
Accession NZ_CP080370
Organism Escherichia coli strain PK5086

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag K0H55_RS03215 Protein ID WP_000347251.1
Coordinates 656169..656633 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag K0H55_RS03220 Protein ID WP_001307405.1
Coordinates 656633..656968 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0H55_RS03185 (651170) 651170..651604 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
K0H55_RS03190 (651622) 651622..652500 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
K0H55_RS03195 (652490) 652490..653269 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
K0H55_RS03200 (653280) 653280..653753 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
K0H55_RS03205 (653776) 653776..655056 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
K0H55_RS03210 (655305) 655305..656114 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
K0H55_RS03215 (656169) 656169..656633 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
K0H55_RS03220 (656633) 656633..656968 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
K0H55_RS03225 (657117) 657117..658688 - 1572 WP_001273761.1 galactarate dehydratase -
K0H55_RS03230 (659063) 659063..660397 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
K0H55_RS03235 (660413) 660413..661183 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T211798 WP_000347251.1 NZ_CP080370:c656633-656169 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T211798 NZ_CP106771:c1659209-1659027 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT211798 WP_001307405.1 NZ_CP080370:c656968-656633 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211798 NZ_CP106771:c1658925-1658524 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References