Detailed information of TA system TA211794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6073414..6074009
• Replicon: chromosome
• Accession: NZ_CP080369
• Organism: Pseudomonas aeruginosa SG17M

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: K0E51_RS28345
• Protein ID: WP_003113526.1
• Coordinates: 6073731..6074009 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K0E51_RS28340
• Protein ID: WP_003113527.1
• Coordinates: 6073414..6073719 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E51_RS28305 (K0E51_28305)	6068553..6069401	+	849	WP_009875808.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
K0E51_RS28315 (K0E51_28315)	6069568..6070509	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
K0E51_RS28320 (K0E51_28320)	6070626..6071240	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
K0E51_RS28325 (K0E51_28325)	6071282..6071866	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
K0E51_RS28330 (K0E51_28330)	6071907..6073007	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
K0E51_RS28340 (K0E51_28340)	6073414..6073719	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
K0E51_RS28345 (K0E51_28345)	6073731..6074009	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K0E51_RS28350	6074062..6074190	-	129	Protein_5617	integrase	-
K0E51_RS28355 (K0E51_28350)	6074338..6076566	+	2229	WP_003113525.1	TonB-dependent receptor	-
K0E51_RS28360 (K0E51_28355)	6076636..6077283	-	648	WP_003095021.1	carbonate dehydratase	-
K0E51_RS28365 (K0E51_28360)	6077345..6078583	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T211794 WP_003113526.1 NZ_CP080369:c6074009-6073731 [Pseudomonas aeruginosa SG17M]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T211794 NZ_CP106769:c950666-950247 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT211794 WP_003113527.1 NZ_CP080369:c6073719-6073414 [Pseudomonas aeruginosa SG17M]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT211794 NZ_CP106769:c950902-950666 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3