Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3085518..3086293 Replicon chromosome
Accession NZ_CP080369
Organism Pseudomonas aeruginosa SG17M

Toxin (Protein)


Gene name yhaV Uniprot ID V6ADY6
Locus tag K0E51_RS14515 Protein ID WP_009518525.1
Coordinates 3085835..3086293 (+) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A7M2ZSF3
Locus tag K0E51_RS14510 Protein ID WP_023087286.1
Coordinates 3085518..3085835 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0E51_RS14480 (K0E51_14460) 3080842..3081492 - 651 WP_023087281.1 isoprenylcysteine carboxylmethyltransferase family protein -
K0E51_RS14485 (K0E51_14465) 3081489..3081728 - 240 WP_023087282.1 DUF2933 domain-containing protein -
K0E51_RS14490 (K0E51_14470) 3081740..3082141 - 402 WP_023087283.1 hypothetical protein -
K0E51_RS14495 3082296..3082730 - 435 WP_078452152.1 hypothetical protein -
K0E51_RS14500 (K0E51_14480) 3082803..3083297 + 495 WP_031633853.1 MerR family DNA-binding protein -
K0E51_RS14505 (K0E51_14485) 3083353..3085218 - 1866 WP_023087285.1 MobH family relaxase -
K0E51_RS14510 (K0E51_14490) 3085518..3085835 + 318 WP_023087286.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
K0E51_RS14515 (K0E51_14495) 3085835..3086293 + 459 WP_009518525.1 type II toxin-antitoxin system YhaV family toxin Toxin
K0E51_RS14520 (K0E51_14500) 3086320..3086679 + 360 WP_023087287.1 DUF3742 family protein -
K0E51_RS14525 (K0E51_14505) 3086686..3088209 - 1524 WP_023087288.1 conjugal transfer protein TraG N-terminal domain-containing protein -
K0E51_RS14530 (K0E51_14510) 3088223..3088582 - 360 WP_023087289.1 hypothetical protein -
K0E51_RS14535 (K0E51_14515) 3088579..3089973 - 1395 WP_023087290.1 integrating conjugative element protein -
K0E51_RS14540 (K0E51_14520) 3089983..3090930 - 948 WP_023087291.1 TIGR03756 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3032231..3147438 115207


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-100)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17660.02 Da        Isoelectric Point: 10.1848

>T211789 WP_009518525.1 NZ_CP080369:3085835-3086293 [Pseudomonas aeruginosa SG17M]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

>T211789 NZ_CP106765:c1654160-1653978 [Neisseria gonorrhoeae]
TTGAATAGCCTAGACGTTATTGCCCTACTCAAACAAGACGGTTGGTATAAAGTTGCACAATCCGGGAGCCATTCGCAATA
TAAGCACCCAACAAAAAAAGGCCGTGTAACCGTGCCGCACCCGAAAAAAGACTTGCCGACAGGTACTGTAAAAAATATCT
ATAAGCAAGCCGGTTTGAAGTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11156.69 Da        Isoelectric Point: 4.5685

>AT211789 WP_023087286.1 NZ_CP080369:3085518-3085835 [Pseudomonas aeruginosa SG17M]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNLQGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Download         Length: 318 bp

>AT211789 NZ_CP106765:c1653876-1653475 [Neisseria gonorrhoeae]
ATGTTTATCCCTGCCGCCCTGCACAAAGACGAACATTCGGCATATGGCGTAACCATCCCCGACCTTCCCGGCTGTTTCTC
TTGCGGCGACACCGTTGAAGAAGCCGTCGCCAACGCCCGTTCTGCCGCCTATATGCATATTGACGGTATGATTGAGGACG
GAGGGTTCAAAAACCTTGCCGTAAGCAGCATTGCCGATTTGAGCCAAGAACCTGATTATCATGGGGCAACATGGGTAATG
ATTGAAATCGACCCTGCAAAAATCAGTCGGCAGCAAATCCGATTCAACGTCAGCTGGCCGCAATACCTTCTTGATAGAGT
GGATGAATATACATCCGCAAATCATGAAACCCGTAGCGGTTTTTTAGCCAAAGCCGCCCTGCTCACCATGAATCAGGCAT
AG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ADY6

Antitoxin

Source ID Structure
AlphaFold DB A0A7M2ZSF3

References