Detailed information of TA system TA211783

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51332..51596
• Replicon: plasmid pCMY2-191773
• Accession: NZ_CP080365
• Organism: Klebsiella pneumoniae strain K191773

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: K0P53_RS28120
• Protein ID: WP_001387489.1
• Coordinates: 51444..51596 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	51332..51392 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0P53_RS28095 (46924)	46924..47241	+	318	WP_000118520.1	quaternary ammonium compound efflux SMR transporter SugE	-
K0P53_RS28100 (47238)	47238..47771	-	534	WP_001221666.1	lipocalin family protein	-
K0P53_RS28105 (47865)	47865..49010	-	1146	WP_000976514.1	class C beta-lactamase CMY-2	-
K0P53_RS28110 (49334)	49334..50596	-	1263	WP_000608644.1	IS1380-like element ISEcp1 family transposase	-
K0P53_RS28115 (50749)	50749..51123	-	375	WP_223349261.1	hypothetical protein	-
- (51332)	51332..51392	-	61	NuclAT_0	-	Antitoxin
- (51332)	51332..51392	-	61	NuclAT_0	-	Antitoxin
- (51332)	51332..51392	-	61	NuclAT_0	-	Antitoxin
- (51332)	51332..51392	-	61	NuclAT_0	-	Antitoxin
K0P53_RS28120 (51444)	51444..51596	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
K0P53_RS28125 (51586)	51586..51798	-	213	WP_050485428.1	hypothetical protein	-
K0P53_RS28130 (52098)	52098..52394	+	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
K0P53_RS28135 (52459)	52459..52635	-	177	WP_001054897.1	hypothetical protein	-
K0P53_RS28140 (53027)	53027..53236	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
K0P53_RS28145 (53308)	53308..53970	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
K0P53_RS28150 (54041)	54041..56209	-	2169	WP_023518847.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..95539	95539
	-	flank	IS/Tn	blaCMY-2	-	47865..50596	2731

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T211783 WP_001387489.1 NZ_CP080365:51444-51596 [Klebsiella pneumoniae]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T211783 NZ_CP106761:c13599-13156 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT211783 NZ_CP080365:c51392-51332 [Klebsiella pneumoniae]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin