Detailed information of TA system TA211723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 33418..33934
• Replicon: plasmid p2
• Accession: NZ_CP080338
• Organism: Lacticaseibacillus paracasei strain L1

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A243Q0H7
• Locus tag: KZR06_RS15490
• Protein ID: WP_003573774.1
• Coordinates: 33653..33934 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: KZR06_RS15485
• Protein ID: WP_016363783.1
• Coordinates: 33418..33660 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZR06_RS15470 (KZR06_15405)	29411..30529	-	1119	WP_259912446.1	hypothetical protein	-
KZR06_RS15475 (KZR06_15410)	30538..32145	-	1608	WP_259912447.1	AAA family ATPase	-
KZR06_RS15480 (KZR06_15415)	32626..33093	-	468	WP_259912448.1	hypothetical protein	-
KZR06_RS15485 (KZR06_15420)	33418..33660	+	243	WP_016363783.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KZR06_RS15490 (KZR06_15425)	33653..33934	+	282	WP_003573774.1	type II toxin-antitoxin system YafQ family toxin	Toxin
KZR06_RS15495 (KZR06_15430)	34187..34810	+	624	WP_259912418.1	zinc ribbon domain-containing protein	-
KZR06_RS15500 (KZR06_15435)	34852..36270	-	1419	WP_052915958.1	IS5-like element ISLrh3 family transposase	-
KZR06_RS15505 (KZR06_15440)	36540..37703	-	1164	WP_259912419.1	PrsW family intramembrane metalloprotease	-
KZR06_RS15510 (KZR06_15445)	37790..38374	-	585	WP_259912420.1	recombinase family protein	-
KZR06_RS15515 (KZR06_15450)	38461..38610	-	150	WP_228772279.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65798	65798
	-	flank	IS/Tn	-	-	34852..36270	1418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (6-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11371.98 Da
• Isoelectric Point: 9.5081

>T211723 WP_003573774.1 NZ_CP080338:33653-33934 [Lacticaseibacillus paracasei]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN

Nucleotide

• Length: 282 bp

>T211723 NZ_CP106752:1692999-1693286 [Psychrobacter sp. SC65A.3]
ATGACTTATAATCTTGAGTTTCATCCTTTAGCATTAAAAGAATGGAAAAAGTTAGCGCCCAGTATTCAGCAGCAGTTTAA
GAAAAAATTACAGCAGCGATTGTCAAACCCTCATGTGCCTGCGTCAAAACTATCGGGACATACGGATACTTATAAAATCA
AATTACGCACCGTAGGCTATCGTCTGGTATATACCGTAAAAGATGATGTGGTGGTAGTTTATGTATTAGCAGTAGGCAAA
AGAGAAAACAACAAAGTATATGACAATCTTGCGACACGCCAGCCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9217.44 Da
• Isoelectric Point: 5.1548

>AT211723 WP_016363783.1 NZ_CP080338:33418-33660 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE

Nucleotide

• Length: 243 bp

>AT211723 NZ_CP106752:1692758-1693009 [Psychrobacter sp. SC65A.3]
ATGCAACCAATATTTGCCACACAGACAGCCAGTATCTCAGAATTAAAGGCCAACCCATCGGCACTGATTAAACAGTCAGA
TGGTGAATCCATTGCTATTTTAAACCACAATAAACCAGTGGCTTACTTAGTCTCAACAGACGTCTTTGAGCGCATGATGG
AAGCGATGGACGACATGGCATTAAGCCAAACCGTTAGCGCACGGTTAAATGATGGGCAGATACCGATAAAGGTAACATTG
GATGACTTATAA

Structures

Toxin

• AlphaFold DB: A0A243Q0H7