Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | DinJ-YafQ (relBE)/YafQ-RelB |
| Location | 23406..23922 | Replicon | plasmid p1 |
| Accession | NZ_CP080337 | ||
| Organism | Lacticaseibacillus paracasei strain L1 | ||
Toxin (Protein)
| Gene name | yafQ | Uniprot ID | A0A243Q0H7 |
| Locus tag | KZR06_RS15045 | Protein ID | WP_003573774.1 |
| Coordinates | 23406..23687 (-) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | dinJ | Uniprot ID | - |
| Locus tag | KZR06_RS15050 | Protein ID | WP_016363783.1 |
| Coordinates | 23680..23922 (-) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KZR06_RS15005 (KZR06_14930) | 19002..19091 | + | 90 | Protein_25 | recombinase family protein | - |
| KZR06_RS15010 (KZR06_14935) | 19170..19565 | + | 396 | WP_259912409.1 | transposase | - |
| KZR06_RS15015 (KZR06_14940) | 19562..20317 | + | 756 | WP_259912410.1 | IS3 family transposase | - |
| KZR06_RS15020 (KZR06_14945) | 20760..21158 | - | 399 | WP_003563273.1 | pyridoxamine 5'-phosphate oxidase family protein | - |
| KZR06_RS15025 (KZR06_14950) | 21272..21643 | - | 372 | WP_003563277.1 | TIGR02328 family protein | - |
| KZR06_RS15030 (KZR06_14955) | 21790..22299 | - | 510 | WP_259912411.1 | alcohol dehydrogenase | - |
| KZR06_RS15035 (KZR06_14960) | 22329..22613 | - | 285 | WP_016363781.1 | BRCT domain-containing protein | - |
| KZR06_RS15040 (KZR06_14965) | 22619..23269 | - | 651 | WP_003586220.1 | recombinase family protein | - |
| KZR06_RS15045 (KZR06_14970) | 23406..23687 | - | 282 | WP_003573774.1 | type II toxin-antitoxin system YafQ family toxin | Toxin |
| KZR06_RS15050 (KZR06_14975) | 23680..23922 | - | 243 | WP_016363783.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | Antitoxin |
| KZR06_RS15055 (KZR06_14980) | 24049..24417 | - | 369 | WP_003582572.1 | YxeA family protein | - |
| KZR06_RS15060 (KZR06_14985) | 25225..25313 | + | 89 | Protein_36 | IS30 family transposase | - |
| KZR06_RS15065 (KZR06_14990) | 25355..25822 | - | 468 | WP_003582565.1 | DNA starvation/stationary phase protection protein | - |
| KZR06_RS15070 (KZR06_14995) | 26243..27085 | - | 843 | WP_015975057.1 | IS3 family transposase | - |
| KZR06_RS15075 (KZR06_15000) | 27139..27390 | - | 252 | WP_002816285.1 | IS3 family transposase | - |
| KZR06_RS15080 (KZR06_15005) | 27487..27705 | + | 219 | Protein_40 | DDE-type integrase/transposase/recombinase | - |
| KZR06_RS15085 (KZR06_15010) | 27799..28098 | - | 300 | WP_259912412.1 | hypothetical protein | - |
| KZR06_RS15090 (KZR06_15015) | 28049..28393 | - | 345 | WP_016376036.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..74549 | 74549 | |
| - | flank | IS/Tn | - | - | 19170..19565 | 395 | |
| - | flank | IS/Tn | - | - | 27139..27390 | 251 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11371.98 Da Isoelectric Point: 9.5081
>T211722 WP_003573774.1 NZ_CP080337:c23687-23406 [Lacticaseibacillus paracasei]
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN
MNKFVYKPAFERQFKKHYKTILKGGRYKKEDFEAVYHKLLRDEPLDPHYNDHALVNRKPERDLHIKPDWLLIYKYDGEFV
RFIDTGSHSDLFN
Download Length: 282 bp
>T211722 NZ_CP106752:c898281-897934 [Psychrobacter sp. SC65A.3]
ATGTGGACTGTCATCACAACAGAGCTGTTCAACCAATGGTTTGATCAGCAAGACGATTCAACGCAAGAAAAAGTGCTGGC
AGCTTTGATGGTTTTAGAACAGCAAGGTCCAAGCTTAGGTCGCCCTTTAGTAGATACGGTGTATGATTCAAAATTTACCA
ATATGAAAGAGTTGCGTGTTCAGCACAGAGGCAAGCCACTCAGAGCCTTTTTTGCCTTTGATCCAAAGCGGCAAGCGATT
GTGCTTTGTATCGGTGATAAAGGTAATAAAAAGCGTTTTTATAAAGAAATGCTAGCCACTGCAGACCAGCAGTATGAGTG
TCACCTCAGAACCTTAGGAGATCCATAA
ATGTGGACTGTCATCACAACAGAGCTGTTCAACCAATGGTTTGATCAGCAAGACGATTCAACGCAAGAAAAAGTGCTGGC
AGCTTTGATGGTTTTAGAACAGCAAGGTCCAAGCTTAGGTCGCCCTTTAGTAGATACGGTGTATGATTCAAAATTTACCA
ATATGAAAGAGTTGCGTGTTCAGCACAGAGGCAAGCCACTCAGAGCCTTTTTTGCCTTTGATCCAAAGCGGCAAGCGATT
GTGCTTTGTATCGGTGATAAAGGTAATAAAAAGCGTTTTTATAAAGAAATGCTAGCCACTGCAGACCAGCAGTATGAGTG
TCACCTCAGAACCTTAGGAGATCCATAA
Antitoxin
Download Length: 81 a.a. Molecular weight: 9217.44 Da Isoelectric Point: 5.1548
>AT211722 WP_016363783.1 NZ_CP080337:c23922-23680 [Lacticaseibacillus paracasei]
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE
MTSQVHFRMSEKEKKDFEIVLSRVGLTPGEAFRIFAKKSIEAGGIPFEVSQPNTRLKKAIRSQDYVEFNDPEEGLDWLNE
Download Length: 243 bp
>AT211722 NZ_CP106752:c897932-897630 [Psychrobacter sp. SC65A.3]
ATGACTAAGACACTACAAGAGATGTTAGCTGAGCGCTCTTTAGATAGCCGAGCGCGTATTAAACAACTGGCAGAGCAGTT
GCTTTTAGAAAACCAGCTGTCTCGTATCCGTGAAGAGTTAGAGATCTCTCAAAAAGAGCTGGCTCAAACCATGGGTATTA
AACAGCCTTCATTGTCTGCTATAGAAAATCGTGGCAATGATCTTAAAATATCGACCATGAAAAAATATGTTGAGGCCATG
GGTGGTAAGCTTCGTATCGACGTTGAGCTTCCAACAGGGAAGCACATAGGCTTTAATGTGTAA
ATGACTAAGACACTACAAGAGATGTTAGCTGAGCGCTCTTTAGATAGCCGAGCGCGTATTAAACAACTGGCAGAGCAGTT
GCTTTTAGAAAACCAGCTGTCTCGTATCCGTGAAGAGTTAGAGATCTCTCAAAAAGAGCTGGCTCAAACCATGGGTATTA
AACAGCCTTCATTGTCTGCTATAGAAAATCGTGGCAATGATCTTAAAATATCGACCATGAAAAAATATGTTGAGGCCATG
GGTGGTAAGCTTCGTATCGACGTTGAGCTTCCAACAGGGAAGCACATAGGCTTTAATGTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|