Detailed information of TA system TA211699

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127832..128085
• Replicon: plasmid p426-KPC
• Accession: NZ_CP080311
• Organism: Klebsiella pneumoniae strain KP-426

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: KZ481_RS28870
• Protein ID: WP_001312851.1
• Coordinates: 127936..128085 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	127832..127891 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ481_RS28830 (123214)	123214..123558	-	345	Protein_156	IS6-like element IS26 family transposase	-
KZ481_RS28835 (123610)	123610..124314	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KZ481_RS28840 (124339)	124339..124539	+	201	WP_072354025.1	hypothetical protein	-
KZ481_RS28845 (124559)	124559..125305	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
KZ481_RS28850 (125360)	125360..125920	+	561	WP_000139328.1	fertility inhibition protein FinO	-
KZ481_RS28855 (126052)	126052..126252	+	201	WP_015059022.1	hypothetical protein	-
KZ481_RS28860 (126638)	126638..127237	+	600	WP_032083981.1	PIN domain-containing protein	-
KZ481_RS28865 (127299)	127299..127631	+	333	WP_152916585.1	hypothetical protein	-
- (127832)	127832..127891	-	60	NuclAT_1	-	Antitoxin
- (127832)	127832..127891	-	60	NuclAT_1	-	Antitoxin
- (127832)	127832..127891	-	60	NuclAT_1	-	Antitoxin
- (127832)	127832..127891	-	60	NuclAT_1	-	Antitoxin
KZ481_RS28870 (127936)	127936..128085	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
KZ481_RS28875 (128369)	128369..128617	+	249	WP_000083837.1	replication regulatory protein RepA	-
KZ481_RS29930 (128732)	128732..128916	+	185	Protein_166	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..128928	128928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T211699 WP_001312851.1 NZ_CP080311:127936-128085 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T211699 NZ_CP106743:c4497350-4496595 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT211699 NZ_CP080311:c127891-127832 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin