Detailed information of TA system TA211665

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30205..30474
• Replicon: plasmid pCT77-KPC
• Accession: NZ_CP080305
• Organism: Klebsiella pneumoniae strain KP-CT77

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: K0E63_RS28265
• Protein ID: WP_001372321.1
• Coordinates: 30349..30474 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30205..30270 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E63_RS28240	25915..26442	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
K0E63_RS28245	26500..26733	+	234	WP_000006003.1	DUF905 family protein	-
K0E63_RS28250	26794..28817	+	2024	Protein_37	ParB/RepB/Spo0J family partition protein	-
K0E63_RS28255	28886..29320	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
K0E63_RS28260	29317..30036	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	30048..30272	+	225	NuclAT_0	-	-
-	30048..30272	+	225	NuclAT_0	-	-
-	30048..30272	+	225	NuclAT_0	-	-
-	30048..30272	+	225	NuclAT_0	-	-
-	30205..30270	-	66	-	-	Antitoxin
K0E63_RS29910	30258..30407	+	150	Protein_40	plasmid maintenance protein Mok	-
K0E63_RS28265	30349..30474	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
K0E63_RS28270	30793..31089	-	297	Protein_42	hypothetical protein	-
K0E63_RS28275	31389..31685	+	297	WP_001272251.1	hypothetical protein	-
K0E63_RS28280	31796..32617	+	822	WP_001234445.1	DUF932 domain-containing protein	-
K0E63_RS28285	32914..33561	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
K0E63_RS28290	33838..34221	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
K0E63_RS28295	34502..35206	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..128928	128928

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T211665 WP_001372321.1 NZ_CP080305:30349-30474 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211665 NZ_CP106681:895883-896638 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT211665 NZ_CP080305:c30270-30205 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin