Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 30992..31719 Replicon plasmid pCT77-Vir
Accession NZ_CP080304
Organism Klebsiella pneumoniae strain KP-CT77

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag K0E63_RS27205 Protein ID WP_011251285.1
Coordinates 31408..31719 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag K0E63_RS27200 Protein ID WP_011251286.1
Coordinates 30992..31411 (-) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K0E63_RS27170 (K0E63_27170) 26160..26630 - 471 WP_048333570.1 hypothetical protein -
K0E63_RS27175 (K0E63_27175) 26943..27578 - 636 WP_223171879.1 hypothetical protein -
K0E63_RS27180 (K0E63_27180) 27997..28617 + 621 WP_223175004.1 conjugative transfer protein MobI(A/C) -
K0E63_RS27185 (K0E63_27185) 28638..29426 + 789 WP_040217257.1 hypothetical protein -
K0E63_RS27190 (K0E63_27190) 29440..29805 + 366 WP_048333448.1 hypothetical protein -
K0E63_RS27195 (K0E63_27195) 29877..30845 - 969 WP_074428168.1 IS5 family transposase -
K0E63_RS27200 (K0E63_27200) 30992..31411 - 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
K0E63_RS27205 (K0E63_27205) 31408..31719 - 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
K0E63_RS27210 (K0E63_27210) 31924..32361 - 438 Protein_39 DDE-type integrase/transposase/recombinase -
K0E63_RS27215 (K0E63_27215) 32496..33193 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
K0E63_RS27220 (K0E63_27220) 34579..35229 - 651 WP_068893702.1 DUF1173 family protein -
K0E63_RS29860 35262..35528 - 267 WP_223175074.1 DUF1173 family protein -
K0E63_RS27225 (K0E63_27225) 35706..36200 + 495 WP_004212794.1 thermonuclease family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..186446 186446
- inside IScluster/Tn - - 23628..39452 15824


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T211663 WP_011251285.1 NZ_CP080304:c31719-31408 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T211663 NZ_CP106680:c4668865-4668500 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGATCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT211663 WP_011251286.1 NZ_CP080304:c31411-30992 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT211663 NZ_CP106680:c4668507-4668184 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGGACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAAATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAACTGGAAGTGATTGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References