Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 40317..41044 | Replicon | plasmid pC76-Vir |
| Accession | NZ_CP080298 | ||
| Organism | Klebsiella pneumoniae strain KP-C76 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | K0E64_RS27220 | Protein ID | WP_011251285.1 |
| Coordinates | 40733..41044 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | K0E64_RS27215 | Protein ID | WP_011251286.1 |
| Coordinates | 40317..40736 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K0E64_RS27185 (K0E64_27185) | 35485..35955 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| K0E64_RS27190 (K0E64_27190) | 36268..36903 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| K0E64_RS27195 (K0E64_27195) | 37322..37942 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| K0E64_RS27200 (K0E64_27200) | 37963..38751 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| K0E64_RS27205 (K0E64_27205) | 38765..39130 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| K0E64_RS27210 (K0E64_27210) | 39202..40170 | - | 969 | WP_074428168.1 | IS5 family transposase | - |
| K0E64_RS27215 (K0E64_27215) | 40317..40736 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| K0E64_RS27220 (K0E64_27220) | 40733..41044 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| K0E64_RS27225 (K0E64_27225) | 41249..41686 | - | 438 | Protein_46 | DDE-type integrase/transposase/recombinase | - |
| K0E64_RS27230 (K0E64_27230) | 41821..42518 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| K0E64_RS27235 (K0E64_27235) | 43904..44554 | - | 651 | WP_068893702.1 | DUF1173 family protein | - |
| K0E64_RS29670 | 44587..44853 | - | 267 | WP_223175074.1 | DUF1173 family protein | - |
| K0E64_RS27240 (K0E64_27240) | 45031..45525 | + | 495 | WP_004212794.1 | thermonuclease family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..197407 | 197407 | |
| - | inside | IScluster/Tn | - | - | 22688..47711 | 25023 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T211634 WP_011251285.1 NZ_CP080298:c41044-40733 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T211634 NZ_CP106658:c5327170-5326781 [Klebsiella michiganensis]
ATGCAAACCAAATCATCCCCTTTAATGCGGGAGGCATCGTCACGTCCGTCTCCCGTTGATGTCTGGCAGGCTCTGCTGAC
CTGCCTGCTGGCACACCACTATGGCCTTACGCTTAATGACACGCCGTTCAGTGATGAACAGGTGATTCAGCAACATATTG
ATGCCGGTATCTCGCTGGTTGATGCCCTGAATTTCATTGTTGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATTACGGCCATTGATATTCTCCGGGCCAGAAAGGCTACAGGGCTGATGAACCGTGG
CACGTATAAAGAGGTGACAGCCATTACCCGGGGACAGTATCCGCAGGCACGCATCTCAGGCAAGCGATGA
ATGCAAACCAAATCATCCCCTTTAATGCGGGAGGCATCGTCACGTCCGTCTCCCGTTGATGTCTGGCAGGCTCTGCTGAC
CTGCCTGCTGGCACACCACTATGGCCTTACGCTTAATGACACGCCGTTCAGTGATGAACAGGTGATTCAGCAACATATTG
ATGCCGGTATCTCGCTGGTTGATGCCCTGAATTTCATTGTTGAAAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTC
AGCATCAGGGAGCAGTCTCCGTTTATTACGGCCATTGATATTCTCCGGGCCAGAAAGGCTACAGGGCTGATGAACCGTGG
CACGTATAAAGAGGTGACAGCCATTACCCGGGGACAGTATCCGCAGGCACGCATCTCAGGCAAGCGATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT211634 WP_011251286.1 NZ_CP080298:c40736-40317 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT211634 NZ_CP106658:c5327553-5327227 [Klebsiella michiganensis]
ATCAGCGAACCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTTTGGGGCCCGTCTGGTCCAGGAGGGAAATCGCCT
GCATTACCTGGCTGACCGGGCAAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATCCCCGTCATCAGCACGGCGTCACACTGTATGCA
AAAGGTCTGACCTGCGAGGCCGATACCCTTGGTTCGCACGGCTACGTTTATATCGCGATTTATCCCGCTCCCGTCACCAC
AGAATAA
ATCAGCGAACCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTTTGGGGCCCGTCTGGTCCAGGAGGGAAATCGCCT
GCATTACCTGGCTGACCGGGCAAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATCCCCGTCATCAGCACGGCGTCACACTGTATGCA
AAAGGTCTGACCTGCGAGGCCGATACCCTTGGTTCGCACGGCTACGTTTATATCGCGATTTATCCCGCTCCCGTCACCAC
AGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|