Detailed information of TA system TA211614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/-
• Location: 2461231..2461894
• Replicon: chromosome
• Accession: NZ_CP080296
• Organism: Bacteroides fragilis strain IHMA_13

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: A0A4Q0U3J6
• Locus tag: K0E65_RS09820
• Protein ID: WP_032536341.1
• Coordinates: 2461559..2461894 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: K0E65_RS09815
• Protein ID: WP_195316616.1
• Coordinates: 2461231..2461557 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E65_RS09790 (K0E65_09775)	2457015..2458328	+	1314	WP_195316618.1	UDP-glucose/GDP-mannose dehydrogenase family protein	-
K0E65_RS09795 (K0E65_09780)	2458331..2459293	+	963	WP_195316633.1	NAD-dependent epimerase/dehydratase family protein	-
K0E65_RS09800 (K0E65_09785)	2459297..2460247	+	951	WP_195316617.1	glycosyltransferase family 4 protein	-
K0E65_RS09805 (K0E65_09790)	2460250..2460834	+	585	WP_229080718.1	N-acetylmuramidase family protein	-
K0E65_RS09810 (K0E65_09795)	2461019..2461234	+	216	WP_032536343.1	helix-turn-helix transcriptional regulator	-
K0E65_RS09815 (K0E65_09800)	2461231..2461557	+	327	WP_195316616.1	HipA N-terminal domain-containing protein	Antitoxin
K0E65_RS09820 (K0E65_09805)	2461559..2461894	+	336	WP_032536341.1	HipA domain-containing protein	Toxin
K0E65_RS09825 (K0E65_09810)	2461942..2463516	-	1575	WP_005776334.1	Rne/Rng family ribonuclease	-
K0E65_RS09830 (K0E65_09815)	2463796..2464071	-	276	WP_005776335.1	HU family DNA-binding protein	-
K0E65_RS09835 (K0E65_09820)	2464276..2465322	+	1047	WP_122130494.1	A/G-specific adenine glycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (1-82)

Antitoxin

• HipA, N-terminal subdomain 1 (5-98)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 13038.10 Da
• Isoelectric Point: 9.2916

>T211614 WP_032536341.1 NZ_CP080296:2461559-2461894 [Bacteroides fragilis]
MHLKNFSLYSTKPISYVLTPAYDLLSTKLVLPADSEELALTLNGKKKKIKKSDFVVAMNSTGLEDKIIENVFNKFDHLQS
KWEEFIDVSFIQETTKERYKELIHENWKRIK

Nucleotide

• Length: 336 bp

>T211614 NZ_CP106654:3987091-3987309 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATTCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12153.92 Da
• Isoelectric Point: 4.1963

>AT211614 WP_195316616.1 NZ_CP080296:2461231-2461557 [Bacteroides fragilis]
MKKAEIYVSNQLAGILIEDENGFSFTYTDEYISKGNRRPVSLTLPITPVSYTSSVLFPFFDGLIPEGWLLDIAEQSWKIS
SRDRMALLLACCKDCIGDSTLLLDYGEF

Nucleotide

• Length: 327 bp

>AT211614 NZ_CP106654:3986690-3987064 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A4Q0U3J6