Detailed information of TA system TA211603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 799565..800160
• Replicon: chromosome
• Accession: NZ_CP080289
• Organism: Pseudomonas aeruginosa strain PA2207

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: K0E62_RS03810
• Protein ID: WP_003113526.1
• Coordinates: 799565..799843 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K0E62_RS03815
• Protein ID: WP_003099268.1
• Coordinates: 799855..800160 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K0E62_RS03795 (K0E62_03795)	794991..796229	+	1239	WP_003113524.1	dipeptidase	-
K0E62_RS03800 (K0E62_03800)	796291..796938	+	648	WP_003095021.1	carbonate dehydratase	-
K0E62_RS03805 (K0E62_03805)	797008..799236	-	2229	WP_003099265.1	TonB-dependent receptor	-
K0E62_RS32635	799384..799512	+	129	Protein_755	integrase	-
K0E62_RS03810 (K0E62_03810)	799565..799843	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K0E62_RS03815 (K0E62_03815)	799855..800160	+	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
K0E62_RS03820 (K0E62_03820)	800778..801953	+	1176	WP_071557852.1	reverse transcriptase family protein	-
K0E62_RS03825 (K0E62_03825)	801940..802641	+	702	WP_152959654.1	retron Ec48 family effector membrane protein	-
K0E62_RS03830 (K0E62_03830)	802644..802979	-	336	WP_058175552.1	hypothetical protein	-
K0E62_RS03835 (K0E62_03835)	803426..803698	+	273	WP_058175553.1	hypothetical protein	-
K0E62_RS03840 (K0E62_03840)	803874..804164	+	291	WP_152959653.1	DUF5447 family protein	-
K0E62_RS03845 (K0E62_03845)	804680..805114	+	435	WP_009875803.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T211603 WP_003113526.1 NZ_CP080289:799565-799843 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T211603 NZ_CP104992:2690197-2690565 [Rhizobium sp. CC-CFT758]
ATGATGGTGCGCAGCAATGTGCCCAATCGTGGCGACGTCTTCTGGATCGACCCCAATCCGGTCGCGGGTAGGGAGATAAA
GGACCGGCACCGCTTCGTGGTCATCACACCGCGCGAAATCAATGCGCTCGGCGTGGCGATGACCGTGCCGATCACAAGCG
GGGGCGGTTTCACCCGCAATGTGGGTCTCGCCGTTCCGATTTCGGGTCATGACACGACTGGCGTTGCCGTCTGCAATCAG
GTCCGCAGCTTCGATATCCCGGCAAGAGTCCAGAGCAAGACCGCACGCTACATCGAGACTTTGGATCAGCCGACAATGGA
TGAGATCGTCGCCCGTGTCGTCAGCGCCATCGATCCAGCCGCTGAATAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT211603 WP_003099268.1 NZ_CP080289:799855-800160 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT211603 NZ_CP104992:2689928-2690200 [Rhizobium sp. CC-CFT758]
ATGGCCATGAGCATCACCACAAAGATCAGAAAGCAGGGCGGTGCGGCGATCGTGACCATACCGCCGGCTGTCTTGAAGAC
GATGCAGCTGGAGATCGGCGACCAGCTTTCGTTGGAGATGTCTGAAGGCGAACTGGTGGTGAAGCCGCTCAGGAGTGCCA
AAAGGCGCTACAAGCTTAGCGAACTGCTGGAAGGCAAGGACGCCATGTCCAGGCTGAATGCGGACGTGGCCTGGGCCACC
GAAGGGGATCCGGTCGGGCACGAAATTTCATGA

Structures

Toxin

• AlphaFold DB: V6ALY3