Detailed information of TA system TA021159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-YefM
• Location: 3600547..3601049
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EB_RS23450
• Protein ID: WP_011239255.1
• Coordinates: 3600547..3600813 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: EB_RS23455
• Protein ID: WP_011239256.1
• Coordinates: 3600807..3601049 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS16965	3596028..3596642	-	615	WP_011239249.1	hypothetical protein	-
EB_RS16970	3596720..3597052	-	333	WP_011239250.1	cupin domain-containing protein	-
EB_RS16975	3597061..3597813	-	753	WP_011239251.1	SDR family oxidoreductase	-
EB_RS16980	3598085..3599242	+	1158	WP_011239252.1	PQQ-dependent sugar dehydrogenase	-
EB_RS16985	3599327..3599686	+	360	WP_041646544.1	MbcA/ParS/Xre antitoxin family protein	-
EB_RS16990	3599683..3600435	+	753	WP_011239254.1	RES family NAD+ phosphorylase	-
EB_RS23450	3600547..3600813	-	267	WP_011239255.1	Txe/YoeB family addiction module toxin	Toxin
EB_RS23455	3600807..3601049	-	243	WP_011239256.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EB_RS17000	3601271..3601525	-	255	WP_041646546.1	hypothetical protein	-
EB_RS17005	3601595..3602719	-	1125	WP_011235911.1	ISAs1-like element ISAzo3 family transposase	-
EB_RS24385	3603159..3603425	-	267	WP_157866646.1	hypothetical protein	-
EB_RS17010	3603463..3603765	-	303	WP_041646547.1	hypothetical protein	-
EB_RS24390	3603858..3604433	-	576	WP_157866647.1	hypothetical protein	-
EB_RS23460	3604653..3604886	+	234	WP_083782991.1	phage integrase N-terminal SAM-like domain-containing protein	-
EB_RS17015	3605133..3605618	+	486	WP_011239262.1	acyl-CoA thioesterase	-
EB_RS17020	3605615..3605845	+	231	WP_041646548.1	VF530 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3601595..3602719	1124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10065.71 Da
• Isoelectric Point: 10.0262

>T21159 WP_011239255.1 NC_006513:c3600813-3600547 [Aromatoleum aromaticum EbN1]
VVSWEVVYARQALKDAKKLAASGLKPKAQELLAVLADDPFKNPPPFEKLVGDLAGAYSRRINIQHRIVYEVFANEKTVRV
LRMWTHYE

Nucleotide

• Length: 267 bp

>T21159 NC_006513:c3600813-3600547 [Aromatoleum aromaticum EbN1]
GTGGTAAGTTGGGAAGTCGTTTATGCCAGGCAAGCACTGAAGGATGCAAAGAAACTTGCTGCAAGCGGTCTTAAACCAAA
GGCGCAAGAACTCCTCGCGGTTCTTGCCGACGACCCATTTAAAAATCCACCTCCCTTTGAAAAATTGGTCGGTGATCTTG
CCGGTGCATACTCTCGTCGCATCAATATTCAACACCGCATCGTGTACGAAGTCTTTGCCAACGAGAAAACGGTTCGCGTC
CTGCGTATGTGGACGCACTATGAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8858.20 Da
• Isoelectric Point: 5.8020

>AT21159 WP_011239256.1 NC_006513:c3601049-3600807 [Aromatoleum aromaticum EbN1]
MNTLTASEARANLYRLIDQTVESHEPIIITGKRNSAVLLSAEDWSAIQETLYLLAVPGMRESIKAGMAEPLAKSSKELKW

Nucleotide

• Length: 243 bp

>AT21159 NC_006513:c3601049-3600807 [Aromatoleum aromaticum EbN1]
ATGAACACACTTACCGCCAGCGAAGCCCGGGCAAATCTTTACCGGCTCATTGATCAAACAGTTGAGTCTCATGAGCCAAT
CATCATTACCGGAAAACGCAACAGTGCCGTGCTTCTCTCCGCAGAAGACTGGAGCGCAATTCAGGAAACTCTGTACCTGC
TAGCCGTACCTGGCATGCGTGAGTCCATCAAGGCTGGGATGGCCGAACCTCTCGCAAAAAGCTCGAAGGAGCTTAAGTGG
TAA