Detailed information of TA system TA211588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5499397..5500078
• Replicon: chromosome
• Accession: NZ_CP080285
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KZ796_RS25345
• Protein ID: WP_015503432.1
• Coordinates: 5499713..5500078 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ796_RS25340
• Protein ID: WP_003145733.1
• Coordinates: 5499397..5499720 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ796_RS25315 (KZ796_25305)	5495310..5495948	+	639	WP_003119439.1	hypothetical protein	-
KZ796_RS25320 (KZ796_25310)	5496191..5496526	+	336	WP_003119438.1	TM2 domain-containing protein	-
KZ796_RS25325 (KZ796_25315)	5496700..5497218	+	519	WP_010793873.1	PAAR domain-containing protein	-
KZ796_RS25330 (KZ796_25320)	5497215..5497916	+	702	WP_030046795.1	VRR-NUC domain-containing protein	-
KZ796_RS25335 (KZ796_25325)	5497933..5499021	+	1089	WP_219841497.1	DUF3396 domain-containing protein	-
KZ796_RS25340 (KZ796_25330)	5499397..5499720	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
KZ796_RS25345 (KZ796_25335)	5499713..5500078	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZ796_RS25350 (KZ796_25340)	5500342..5500581	-	240	WP_044060930.1	hypothetical protein	-
KZ796_RS25355 (KZ796_25345)	5500788..5501060	+	273	WP_023104842.1	hypothetical protein	-
KZ796_RS25360 (KZ796_25350)	5501091..5501516	-	426	WP_003085665.1	VOC family protein	-
KZ796_RS25365 (KZ796_25355)	5501618..5502502	+	885	WP_003085663.1	LysR substrate-binding domain-containing protein	-
KZ796_RS25370 (KZ796_25360)	5502475..5503428	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
KZ796_RS25375 (KZ796_25365)	5503649..5504083	+	435	WP_003116494.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T211588 WP_015503432.1 NZ_CP080285:c5500078-5499713 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T211588 NZ_CP104976:c993847-993548 [Enterobacter sp. 155105]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAACGTTATTCCTTCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCCGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGCTATTCCTCAATCCGGGTTAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT211588 WP_003145733.1 NZ_CP080285:c5499720-5499397 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT211588 NZ_CP104976:c993537-993190 [Enterobacter sp. 155105]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTGAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCACAATCTAACTCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA