Detailed information of TA system TA211583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 11652..12247
• Replicon: chromosome
• Accession: NZ_CP080285
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: KZ796_RS00080
• Protein ID: WP_003117425.1
• Coordinates: 11969..12247 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ796_RS00075
• Protein ID: WP_003113527.1
• Coordinates: 11652..11957 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ796_RS00045 (KZ796_00045)	6683..7117	-	435	WP_003140508.1	hypothetical protein	-
KZ796_RS00050 (KZ796_00050)	7633..7922	-	290	Protein_9	DUF5447 family protein	-
KZ796_RS00055 (KZ796_00055)	8098..8370	-	273	WP_058175553.1	hypothetical protein	-
KZ796_RS00060 (KZ796_00060)	8818..9153	+	336	WP_058175552.1	hypothetical protein	-
KZ796_RS00065 (KZ796_00065)	9156..9857	-	702	WP_219841507.1	retron Ec48 family effector membrane protein	-
KZ796_RS00070 (KZ796_00070)	9844..11019	-	1176	WP_071557852.1	reverse transcriptase family protein	-
KZ796_RS00075 (KZ796_00075)	11652..11957	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KZ796_RS00080 (KZ796_00080)	11969..12247	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZ796_RS28135	12300..12428	-	129	Protein_16	integrase	-
KZ796_RS00085 (KZ796_00085)	12576..14804	+	2229	WP_004352671.1	TonB-dependent receptor	-
KZ796_RS00090 (KZ796_00090)	14874..15521	-	648	WP_003095021.1	carbonate dehydratase	-
KZ796_RS00095 (KZ796_00095)	15583..16821	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T211583 WP_003117425.1 NZ_CP080285:c12247-11969 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T211583 NZ_CP104973:2363656-2363937 [Phyllobacterium zundukense]
ATGGTACTAAAAAATGGTACTCAATTTATGAAGCGTAAACACCACATTACCTTGATGCAGATTTTCGCTCGTCCAGTCAG
TGGTTCAGTTCGCTGGGTAGATGTGGAAGCATTGTTTGTCGGTCTCGGTGTTGAAATCAGCGAAAGAGAAGGCTCGCGTG
TCGGTGTCGTTTTATTTGGCGAAGTTCGAGTGTTTCACCGCCCGCATCCATCGCCGGATACCGACAAGGGTGCAATTGCG
AGCATCAGAAAATGGCTGGAAAGCCATGGAGTGAAGCCATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT211583 WP_003113527.1 NZ_CP080285:c11957-11652 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT211583 NZ_CP104973:2363934-2364272 [Phyllobacterium zundukense]
ATGAAAAATGTGATTGATATCGACGGTCACAAAGCGGTCGTTTCGCTTGATCCGGAAATCGGTATGATCCGTGGAGAATT
TCTCGGATTGTCCGGAGGCGCAGATTTTTATGCGAGGAGCGTCGATGATCTGATTGTTGAGGGTCGTAAGTCGCTCAAGG
TTTTTCTTGAGATGTGTGCTGAGAAAGGCATTCCTCCATTCCGCGAATTCTCTGGTCGTTTCAATGTACGCCTTGCTGCG
GAAGTTCACGAAGCTGCCGTCTTGGCAGCTGCTGCAGAAAACAAAAGTCTCAATGAATGGGTAGCCGACGCGATCGAGGC
AGCTGCTCGCGCCGCGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5