Detailed information of TA system TA211577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 449884..450479
• Replicon: chromosome
• Accession: NZ_CP080281
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KZ799_RS02065
• Protein ID: WP_003113526.1
• Coordinates: 450201..450479 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ799_RS02060
• Protein ID: WP_003113527.1
• Coordinates: 449884..450189 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ799_RS02025 (KZ799_02025)	445023..445871	+	849	WP_003123430.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
KZ799_RS02035 (KZ799_02035)	446038..446979	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
KZ799_RS02040 (KZ799_02040)	447096..447710	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
KZ799_RS02045 (KZ799_02045)	447752..448336	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
KZ799_RS02050 (KZ799_02050)	448377..449477	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
KZ799_RS02060 (KZ799_02060)	449884..450189	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
KZ799_RS02065 (KZ799_02065)	450201..450479	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZ799_RS29375	450532..450660	-	129	Protein_405	integrase	-
KZ799_RS02070 (KZ799_02070)	450808..453036	+	2229	WP_023086667.1	TonB-dependent receptor	-
KZ799_RS02075 (KZ799_02075)	453106..453753	-	648	WP_003095021.1	carbonate dehydratase	-
KZ799_RS02080 (KZ799_02080)	453815..455053	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T211577 WP_003113526.1 NZ_CP080281:c450479-450201 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T211577 NZ_CP104972:592170-592553 [Phyllobacterium zundukense]
GTGATCCTCGCGGACACCTCGATCTGGATTGATCATTTTCGTCGTGTTGATGCGGAGCTGCGCAGGATTATTGAAGACGA
CCTCCTACTTTGCCACCCAGCCGTGATCGGTGAGCTGGCGCTTGGCAGCCTTAGGGATCGCGGCAGCGTGATCGCTTTTC
TGGCGGCGCAGCGCGCGGCGGTCGTCGCCACGCACGACGAAGTCATGACAATGATCGATCGCCACAGTCTTTTCAGCATC
GGCATTGGCTACACCGATGCTCACCTGCTGGCATCCGTCCTTCTTGACCAGCGAGCGACCTTATGGACCAGAGACAAGCG
CCTAAGAGCGGCGGCCGAAAAGGCAGGCGCTTCACTGCACCTACCCGTAAATCGTCCGAACTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT211577 WP_003113527.1 NZ_CP080281:c450189-449884 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT211577 NZ_CP104972:591973-592173 [Phyllobacterium zundukense]
ATGCGATCAACTATTAACCTCGACGATACGCTTATGGAGAAGGCCAGGTTCTTGACCGGTACAAATGAAACAGCGGCCCT
AGTGCGCCAGGCGTTGGAAACGCTTGTCCGTGTGGAGTCCGGAAAGCGCCTCATCGCGCTCGGAGGAACTATGCCCGATG
CCGAGGCAGCTCCGCGCCGCCGGAGCGCAGCAGCCAAGTGA

Structures

Toxin

• AlphaFold DB: V6ALY3