Detailed information of TA system TA211569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 811982..812577
• Replicon: chromosome
• Accession: NZ_CP080280
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF20

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: KZ794_RS03645
• Protein ID: WP_003117425.1
• Coordinates: 811982..812260 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ794_RS03650
• Protein ID: WP_003099268.1
• Coordinates: 812272..812577 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ794_RS03630 (KZ794_03630)	807408..808646	+	1239	WP_014603324.1	C69 family dipeptidase	-
KZ794_RS03635 (KZ794_03635)	808708..809355	+	648	WP_003095021.1	carbonate dehydratase	-
KZ794_RS03640 (KZ794_03640)	809425..811653	-	2229	WP_003099265.1	TonB-dependent receptor	-
KZ794_RS31420	811801..811929	+	129	Protein_714	integrase	-
KZ794_RS03645 (KZ794_03645)	811982..812260	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZ794_RS03650 (KZ794_03650)	812272..812577	+	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
KZ794_RS03655 (KZ794_03655)	812954..813481	+	528	WP_071535723.1	ATP-binding protein	-
KZ794_RS03660 (KZ794_03660)	813493..815031	-	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
KZ794_RS03665 (KZ794_03665)	815006..815842	-	837	WP_223656480.1	helix-turn-helix domain-containing protein	-
KZ794_RS03670 (KZ794_03670)	815974..816240	-	267	WP_016852153.1	hypothetical protein	-
KZ794_RS03675 (KZ794_03675)	816350..816622	+	273	WP_003115921.1	hypothetical protein	-
KZ794_RS03680 (KZ794_03680)	816834..817124	+	291	WP_023083237.1	DUF5447 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T211569 WP_003117425.1 NZ_CP080280:811982-812260 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T211569 NZ_CP104967:c352792-352388 [Phyllobacterium sp. A18/5-2]
GTGAGCAAACGCTACATGCTGGACACCAACATCGTGTCCGAGCTGGCGCGCAACCCGCACGGCGCTGTCACCAGACGCAT
CGCCGAAGTTGGGCCGGATGCGATCTGCATCAGCATCATCACGGCCGCAGAGCTGCGATATGGTTGCGCCAAGAAGGGAT
CTCCGAAGCTGCTGGCGCAGATCGAGGCCATCCTTGGGAGCTTGCCAGTGCTAGCGCTCGATGTGCCCGCTGATGCCGAA
TACGGCGGTATCCGTGCCGAACTGGAAGCCGCTGGAAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTA
TGCACTGGGGGCGGTGCTGGTGACGGCCAATACCGGGGAGTTCACGCGCATCCGCGCCCTGCGGATCGAAAACTGGCTGG
GCTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT211569 WP_003099268.1 NZ_CP080280:812272-812577 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT211569 NZ_CP104967:c353058-352789 [Phyllobacterium sp. A18/5-2]
ATGCCCCAACTCCGACACCATGAGCAAGAGCCGCCACGGGAGGCCAAGCTCTTCCGCAACAACAAGAGCCAGGCCGTGCG
TATCCCGGCCGACTTCGAGCTTCCCGGCGACCGGGTGATGATCCATCGGGAGGGTGATCGGCTCATCATCGAGCCGGTGC
GCCGCAAGAACCTGCTGGAAGTACTGGCCGGTCTGGAACCGCTCGGGCGGGCAGATCAGTTCCCTGAGATTGATGATACC
CTGTTGCCGCTGAAGGATATCGACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5