Detailed information of TA system TA211550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 68661..69318
• Replicon: plasmid p135-4
• Accession: NZ_CP080264
• Organism: Escherichia coli strain 135

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: KZ312_RS28440
• Protein ID: WP_000270043.1
• Coordinates: 68661..69011 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ312_RS28445
• Protein ID: WP_000124640.1
• Coordinates: 69016..69318 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ312_RS28395 (KZ312_28395)	63972..64151	+	180	Protein_73	helix-turn-helix domain-containing protein	-
KZ312_RS28400 (KZ312_28400)	64216..64920	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KZ312_RS28405 (KZ312_28405)	65135..65632	-	498	WP_000062185.1	hypothetical protein	-
KZ312_RS28410 (KZ312_28410)	65635..66123	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
KZ312_RS28415 (KZ312_28415)	66220..66555	+	336	WP_000683476.1	hypothetical protein	-
KZ312_RS28420 (KZ312_28420)	66570..67040	-	471	WP_001281821.1	hypothetical protein	-
KZ312_RS28425 (KZ312_28425)	67033..67404	-	372	WP_000516916.1	hypothetical protein	-
KZ312_RS28430 (KZ312_28430)	67415..67609	-	195	WP_000343597.1	hypothetical protein	-
KZ312_RS28435 (KZ312_28435)	67950..68498	-	549	WP_001061574.1	transcriptional regulator	-
KZ312_RS28440 (KZ312_28440)	68661..69011	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KZ312_RS28445 (KZ312_28445)	69016..69318	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
KZ312_RS28450 (KZ312_28450)	69345..69638	-	294	WP_001239997.1	chromosome segregation protein ParM	-
KZ312_RS28455 (KZ312_28455)	69726..69998	-	273	WP_001043047.1	HU family DNA-binding protein	-
KZ312_RS28460 (KZ312_28460)	70056..70583	-	528	WP_001236377.1	thermonuclease family protein	-
KZ312_RS28465 (KZ312_28465)	70814..71671	-	858	WP_001167032.1	hypothetical protein	-
KZ312_RS28470 (KZ312_28470)	71658..71888	-	231	WP_000972663.1	hypothetical protein	-
KZ312_RS28475 (KZ312_28475)	71888..72406	-	519	WP_000210756.1	nitrite reductase	-
KZ312_RS28480 (KZ312_28480)	72403..72849	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
KZ312_RS28485 (KZ312_28485)	72849..73208	-	360	WP_000422768.1	hypothetical protein	-
KZ312_RS28490 (KZ312_28490)	73265..73693	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / aph(3')-Ia / blaCMY-2 / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..113140	113140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T211550 WP_000270043.1 NZ_CP080264:68661-69011 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T211550 NZ_CP104925:2053024-2053377 [Klebsiella pasteurii]
GTGTGGATGATTAAAACAACGGATACGTTTGAGCGCTGGTTTACCTCGCTCAACGATACCGATAGGGCCAGCGTGCTTGC
AGCGCTGTTGGTCCTGCGAGAAAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGCTCAGAGGCTCCCGCTACAGCA
ACATGAAAGAGTTGCGTATTCAGAGCCGGGGCGAGCCGATACGGGCATTTTTCGCTTTTGACCCGGCCCGTACCGGTATT
GTGCTTTGCGCCGGAAATAAGGTCAGCAATGAAAAACGTTTTTACGATGAGATTCTCCCGGTGGCTGACCGGGAATATAC
GAACTGGCTGAACACATTAAAAGAGAAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT211550 WP_000124640.1 NZ_CP080264:69016-69318 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT211550 NZ_CP104925:2053382-2053684 [Klebsiella pasteurii]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAACCTGAAGTTGTGGCCGAAGCCGAGGCTATGGCTAAGGATAT
TTTGCTCAATATTCATCTTGCTGAGTTGCGTGAGAGGGTGCAGAAAACGCAGGTGGAAATGGCGCAGGAATTAGGTATCA
AGCAGCCTACCGTCGCGGGAATGGAAAAACCGGGCCGCGACCTGAAGCTCTCCACGCTCAAACGTTACGTTGAAGCGGCG
GGCGGTAAGCTAAGCCTCAATGTTGAGCTGCCGGATGGGTCGCATTACGGGTTTGTACTGTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9