Detailed information of TA system TA211546

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1163..1580
• Replicon: plasmid p135-3
• Accession: NZ_CP080263
• Organism: Escherichia coli strain 135

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KZ312_RS27360
• Protein ID: WP_096937776.1
• Coordinates: 1455..1580 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	1163..1357 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ312_RS27350 (1)	1..435	+	435	WP_029400524.1	conjugation system SOS inhibitor PsiB	-
KZ312_RS27355 (432)	432..1173	+	742	Protein_1	plasmid SOS inhibition protein A	-
- (1163)	1163..1357	+	195	NuclAT_0	-	Antitoxin
- (1163)	1163..1357	+	195	NuclAT_0	-	Antitoxin
- (1163)	1163..1357	+	195	NuclAT_0	-	Antitoxin
- (1163)	1163..1357	+	195	NuclAT_0	-	Antitoxin
KZ312_RS29220 (1364)	1364..1513	+	150	Protein_2	DUF5431 family protein	-
KZ312_RS27360 (1455)	1455..1580	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZ312_RS27365 (1673)	1673..1837	+	165	WP_001716565.1	hypothetical protein	-
KZ312_RS27370 (2127)	2127..2414	+	288	WP_000107535.1	hypothetical protein	-
KZ312_RS27375 (2533)	2533..3354	+	822	WP_001234469.1	DUF932 domain-containing protein	-
KZ312_RS27380 (3651)	3651..4253	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
KZ312_RS27385 (4574)	4574..4957	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZ312_RS27390 (5144)	5144..5833	+	690	WP_029400181.1	conjugal transfer transcriptional regulator TraJ	-
KZ312_RS27395 (5926)	5926..6327	+	402	WP_001369361.1	conjugal transfer relaxosome DNA-bindin protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papD / papC / papH	1..125082	125082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T211546 WP_096937776.1 NZ_CP080263:1455-1580 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T211546 NZ_CP104920:c3777352-3777077 [Dickeya solani]
ATGAAAGAACAAGTCAGATCATTACGGAAGAGGCAAAAAAGCACGCTTGAACAGGTGTTTAAATCTCCTGTTCAGTCAGG
TATCAAATGGGCCGATATCGAGTCACTGATAAAAGCTCTGGGAGGGGACGTCAAAGAGGGTCGAGGTTCGCGTTGTAAGT
TTCTCCTTAATGGCAGTATCGCCAACTTTCACCGCCCACACCCTTCACCAGATACGGATAAAGGCGCAGTAGCCAACCTG
CGCGACTGGCTTGAAAGCATAGGAGTGAAGCCATGA

Antitoxin

Nucleotide

• Length: 195 bp

>AT211546 NZ_CP080263:1163-1357 [Escherichia coli]
TCACACGGATTACCCGTAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin