Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4930752..4931551 Replicon chromosome
Accession NZ_CP080260
Organism Escherichia coli strain 135

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag KZ312_RS23610 Protein ID WP_000347267.1
Coordinates 4931087..4931551 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KZ312_RS23605 Protein ID WP_001307405.1
Coordinates 4930752..4931087 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ312_RS23590 (4926537) 4926537..4927307 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
KZ312_RS23595 (4927323) 4927323..4928657 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KZ312_RS23600 (4929032) 4929032..4930603 + 1572 WP_001273741.1 galactarate dehydratase -
KZ312_RS23605 (4930752) 4930752..4931087 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KZ312_RS23610 (4931087) 4931087..4931551 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KZ312_RS23615 (4931606) 4931606..4932415 - 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
KZ312_RS23620 (4932664) 4932664..4933944 + 1281 WP_000681922.1 tagatose-bisphosphate aldolase subunit KbaZ -
KZ312_RS23625 (4933967) 4933967..4934440 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KZ312_RS23630 (4934451) 4934451..4935230 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KZ312_RS23635 (4935220) 4935220..4936098 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
KZ312_RS23640 (4936116) 4936116..4936550 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4919877..4931551 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T211543 WP_000347267.1 NZ_CP080260:4931087-4931551 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T211543 NZ_CP104920:c3032446-3032141 [Dickeya solani]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGCCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCCAAGCGGCGCATGGTCATCCCGCTTATCGAAGCACATAACCTGTCTGAGAAAGTGAATAAGACGTTGTTTCCTCTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACGACTGAGCTATCGAGCGTTCCCGTTGAGGTCATTGGTGAGGCTATC
GCGGATCTTGGCGACTACGCAGATGAGATCAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT211543 WP_001307405.1 NZ_CP080260:4930752-4931087 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT211543 NZ_CP104920:c3032667-3032449 [Dickeya solani]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTACCAGGTTCTGAGTGCTGCTGGAGTCAATATTTCCGGGCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGCATCAAGGCTGAGGAGTGGAAGAAAGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACAGCATGGCTCCTTTGCGGATGAAAACAGGAACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References