Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2975937..2976499 Replicon chromosome
Accession NZ_CP080260
Organism Escherichia coli strain 135

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag KZ312_RS14305 Protein ID WP_000605676.1
Coordinates 2976221..2976499 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KZ312_RS14300 Protein ID WP_000781370.1
Coordinates 2975937..2976221 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ312_RS14285 (2971119) 2971119..2974166 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
KZ312_RS14290 (2974179) 2974179..2975063 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
KZ312_RS14295 (2975056) 2975056..2975709 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
KZ312_RS14300 (2975937) 2975937..2976221 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KZ312_RS14305 (2976221) 2976221..2976499 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KZ312_RS14310 (2976685) 2976685..2977695 - 1011 WP_054632500.1 alcohol dehydrogenase AdhP -
KZ312_RS14315 (2977829) 2977829..2979526 - 1698 WP_000433464.1 malate dehydrogenase -
KZ312_RS14320 (2979683) 2979683..2979820 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KZ312_RS14325 (2979922) 2979922..2980137 - 216 WP_000495766.1 biofilm-dependent modulation protein -
KZ312_RS14330 (2980482) 2980482..2980913 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T211529 WP_000605676.1 NZ_CP080260:c2976499-2976221 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T211529 NZ_CP104914:c2140069-2139893 [Avibacterium paragallinarum]
GTGAACAGCAAACAAATGATAAAATTGCTCAAACAAGATGGTTGGTATCTTGATAGCATAAATGGAAGCCACCACCATTT
TGAACATCATCAAAAAAAGGGAAAAGTTACCGTTCCCCACCCAAGAGCCGAATTAGGACATCTTGAAAAATTAATCAGAA
AACAAGCGGGGCTATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT211529 WP_000781370.1 NZ_CP080260:c2976221-2975937 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT211529 NZ_CP104914:c2139871-2139467 [Avibacterium paragallinarum]
ATGTTATATCCAATCGCACTTGAAAAAGTTACTGATGGTTATGTCGTCTCCGTGCCTGATATTCCTGGCTGTTTTTCAGC
TGGTGATACATTGGAAGAGGCATATAACAATGTAAAACAAGCTATCACATCACATCTGGAGTTAGTTGTCCGAGACAATG
AAGAAGTTCCTCTACCAACCCCGCTAGAAGAACATAAAGCCAATCCAGATTATCAAAGCGTTGATATTTTCTTTGGCGTC
ATTGATGTGGACATCAGCCACCTACTTGGCAAAGCCGAACGCATTAACATCACAATGCCCGCTTATTTAATTAAACGCAT
TGATGATTTTGTTGCTACCCATCCGCACTACAAAAGCCGTAGTCACTTTCTTGCCTCGGTTAGCGCAGATAAAATAATGG
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References