Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1372548..1373242 Replicon chromosome
Accession NZ_CP080260
Organism Escherichia coli strain 135

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag KZ312_RS06350 Protein ID WP_001521903.1
Coordinates 1372844..1373242 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag KZ312_RS06345 Protein ID WP_000554758.1
Coordinates 1372548..1372841 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ312_RS06325 (1367965) 1367965..1368462 + 498 WP_000006263.1 REP-associated tyrosine transposase RayT -
KZ312_RS06330 (1368901) 1368901..1370613 - 1713 Protein_1223 flagellar biosynthesis protein FlhA -
KZ312_RS06335 (1370585) 1370585..1371370 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
KZ312_RS06340 (1371441) 1371441..1372496 + 1056 WP_001226177.1 DNA polymerase IV -
KZ312_RS06345 (1372548) 1372548..1372841 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KZ312_RS06350 (1372844) 1372844..1373242 + 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KZ312_RS06355 (1373252) 1373252..1373704 + 453 WP_001059899.1 GNAT family N-acetyltransferase -
KZ312_RS06360 (1373950) 1373950..1374156 + 207 Protein_1229 RtcB family protein -
KZ312_RS06365 (1374152) 1374152..1374504 + 353 Protein_1230 peptide chain release factor H -
KZ312_RS06370 (1374561) 1374561..1376018 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
KZ312_RS06375 (1376279) 1376279..1376737 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (1377333) 1377333..1377413 + 81 NuclAT_9 - -
- (1377333) 1377333..1377413 + 81 NuclAT_9 - -
- (1377333) 1377333..1377413 + 81 NuclAT_9 - -
- (1377333) 1377333..1377413 + 81 NuclAT_9 - -
KZ312_RS06380 (1376829) 1376829..1378073 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T211514 WP_001521903.1 NZ_CP080260:1372844-1373242 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T211514 NZ_CP104908:c36808-36419 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT211514 WP_000554758.1 NZ_CP080260:1372548-1372841 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT211514 NZ_CP104908:c37072-36815 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References