Detailed information of TA system TA211498

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4133..4402
• Replicon: plasmid pSJC33-1
• Accession: NZ_CP080256
• Organism: Escherichia coli strain SJC33

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: KZ313_RS22935
• Protein ID: WP_001323520.1
• Coordinates: 4286..4402 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	4133..4198 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ313_RS22905	2..439	+	438	Protein_0	single-stranded DNA-binding protein	-
KZ313_RS22910	502..735	+	234	WP_000005990.1	DUF905 family protein	-
KZ313_RS22915	801..2759	+	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
KZ313_RS22920	2814..3248	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
KZ313_RS22925	3245..4007	+	763	Protein_4	plasmid SOS inhibition protein A	-
KZ313_RS22930	3976..4164	-	189	WP_001299721.1	hypothetical protein	-
-	3976..4200	+	225	NuclAT_0	-	-
-	3976..4200	+	225	NuclAT_0	-	-
-	3976..4200	+	225	NuclAT_0	-	-
-	3976..4200	+	225	NuclAT_0	-	-
-	4133..4198	-	66	-	-	Antitoxin
KZ313_RS25485	4186..4335	+	150	Protein_6	plasmid maintenance protein Mok	-
KZ313_RS22935	4286..4402	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
KZ313_RS25490	4622..4852	+	231	WP_071587244.1	hypothetical protein	-
KZ313_RS25495	4850..5023	-	174	Protein_9	hypothetical protein	-
KZ313_RS22940	5093..5299	+	207	WP_000547939.1	hypothetical protein	-
KZ313_RS22945	5324..5611	+	288	WP_000107535.1	hypothetical protein	-
KZ313_RS22950	5733..6554	+	822	WP_001234469.1	DUF932 domain-containing protein	-
KZ313_RS22955	6851..7453	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
KZ313_RS22960	7774..8157	+	384	WP_053320747.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KZ313_RS22965	8344..9033	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T211498 WP_001323520.1 NZ_CP080256:4286-4402 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T211498 NZ_CP104882:c128374-128078 [Aggregatibacter actinomycetemcomitans]
ATGAGTGAACAATTGAAAGATTTTGATCCTGCAGAATATTTATCTACCGAAGAAGATATTCGGCTTTATTTAAATGAAGT
GCTACAAGAAAATAATATTGAGCTTATTTTGTCTGCGTTAGGCGATATCGCACGAACCAGAAATATGAGCCAAATTGCCC
GCGAAACCGGTATGAGTAGAGAAGGGCTTTATAAAGCCCTGTCCGGTACGGGCAATCCTACCTTTGCCACCGTGATTAAA
GTCATGAAAGCCTTAAATTTACAACTCCAAGTGCAACCTTCCGGTTATGTTTCTTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT211498 NZ_CP080256:c4198-4133 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin