Detailed information of TA system TA211497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4639621..4640223
• Replicon: chromosome
• Accession: NZ_CP080255
• Organism: Escherichia coli strain SJC33

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KZ313_RS21960
• Protein ID: WP_000897305.1
• Coordinates: 4639912..4640223 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ313_RS21955
• Protein ID: WP_000356395.1
• Coordinates: 4639621..4639911 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ313_RS21920 (4635245)	4635245..4636147	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KZ313_RS21925 (4636144)	4636144..4636779	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KZ313_RS21930 (4636776)	4636776..4637705	+	930	WP_109553726.1	formate dehydrogenase accessory protein FdhE	-
KZ313_RS21935 (4637887)	4637887..4638129	-	243	WP_001309881.1	CopG family transcriptional regulator	-
KZ313_RS21940 (4638348)	4638348..4638566	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KZ313_RS21945 (4638985)	4638985..4639263	-	279	WP_001315112.1	hypothetical protein	-
KZ313_RS21950 (4639315)	4639315..4639536	-	222	WP_001550354.1	hypothetical protein	-
KZ313_RS21955 (4639621)	4639621..4639911	-	291	WP_000356395.1	NadS family protein	Antitoxin
KZ313_RS21960 (4639912)	4639912..4640223	-	312	WP_000897305.1	hypothetical protein	Toxin
KZ313_RS21965 (4640452)	4640452..4641360	+	909	WP_001550353.1	alpha/beta hydrolase	-
KZ313_RS25465 (4641528)	4641528..4642442	-	915	WP_109553727.1	transposase	-
KZ313_RS25470 (4642455)	4642455..4643342	-	888	Protein_4297	hypothetical protein	-
KZ313_RS21975 (4643758)	4643758..4644699	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KZ313_RS21980 (4644744)	4644744..4645181	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T211497 WP_000897305.1 NZ_CP080255:c4640223-4639912 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T211497 NZ_CP104882:90847-91185 [Aggregatibacter actinomycetemcomitans]
ATGGCGAAAGCTCACAAATGGCCGACGGAATTTCAATATCCCCCAAAATGGCTTGATTCTGCGCGCTCCCGTCCAATTTC
ATTATCATTGCCTTTATCCCATAAAGTTTACCGAGAGGATTCGGTTTACAATTTCTTCGATAATCTTTTGCCCGACAATG
AACAAATTCGTTCACGCATTCAGCAACGTTTCCAGACCTCGACCAAATCCCCCTTTGATTTGCTTTCGGCTATTGAGCAG
GATTGCGTTGGGGCGATTCAACTTTATCACGGTGAAAGTCGCTCTGTTCGGCAAATTTATGCGGAGCCGTTAAATGATAA
GAAGTGTAAAACGTGTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT211497 WP_000356395.1 NZ_CP080255:c4639911-4639621 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT211497 NZ_CP104882:90516-90797 [Aggregatibacter actinomycetemcomitans]
ATGGTTATTACCACCGCAAAAATGCTTTCTCATGTTATTCGGGAATTTCGTTATCAAGGCAACCTCTCACAAACAGATGT
CGCCAAGCTTGCTAGCACCACCCAAGCCAGAATATCGGTTTTTGAAAATGAGCCGGAACGCACGAAATTAGAAACGTTAT
TTAAAATTTTGGCAGGATTAGAGTTAGAACTTATCGTACAACCGCGCAAAAAATCCGCGGTTACGGAAAATTTAATCAGC
GAACCAACACCTGCATTTTCCGATGACGATGAGGCGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7