Detailed information of TA system TA211495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3781788..3782482
• Replicon: chromosome
• Accession: NZ_CP080255
• Organism: Escherichia coli strain SJC33

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: KZ313_RS18015
• Protein ID: WP_001263491.1
• Coordinates: 3781788..3782186 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: KZ313_RS18020
• Protein ID: WP_000554755.1
• Coordinates: 3782189..3782482 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3777617)	3777617..3777697	-	81	NuclAT_10	-	-
- (3777617)	3777617..3777697	-	81	NuclAT_10	-	-
- (3777617)	3777617..3777697	-	81	NuclAT_10	-	-
- (3777617)	3777617..3777697	-	81	NuclAT_10	-	-
KZ313_RS17985 (3776957)	3776957..3778201	-	1245	WP_000189541.1	esterase FrsA	-
KZ313_RS17990 (3778293)	3778293..3778751	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KZ313_RS17995 (3779012)	3779012..3780469	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
KZ313_RS18000 (3780526)	3780526..3780878	-	353	Protein_3527	peptide chain release factor H	-
KZ313_RS18005 (3780874)	3780874..3781080	-	207	Protein_3528	RtcB family protein	-
KZ313_RS18010 (3781326)	3781326..3781778	-	453	WP_019842500.1	GNAT family N-acetyltransferase	-
KZ313_RS18015 (3781788)	3781788..3782186	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KZ313_RS18020 (3782189)	3782189..3782482	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KZ313_RS18025 (3782534)	3782534..3783589	-	1056	WP_001550655.1	DNA polymerase IV	-
KZ313_RS18030 (3783660)	3783660..3784445	-	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
KZ313_RS18035 (3784417)	3784417..3786129	+	1713	Protein_3534	flagellar biosynthesis protein FlhA	-
KZ313_RS18040 (3786234)	3786234..3786512	+	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
KZ313_RS18045 (3786505)	3786505..3786861	+	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3771724..3782482	10758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T211495 WP_001263491.1 NZ_CP080255:c3782186-3781788 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T211495 NZ_CP104878:2557900-2558079 [Bacillus subtilis]
ATGTCGACCTATGAATCTCTAATGGTCATGATCGGCTTTGCCAATTTAATAGGCGGGATTATGACATGGGTAATATCTCT
TTTAACATTATTATTCATGCTTAGAAAAAAAGACACTCATCCTATTTACATTACTGTAAAGGAAAAGTGTCTACACGAGG
ACCCTCCTATTAAAGGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT211495 WP_000554755.1 NZ_CP080255:c3782482-3782189 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT211495 NZ_CP104878:c2558225-2558003 [Bacillus subtilis]
CAAGCAAAAGTATTGCAACTACTGGCGTAAGCTTATAGTTGGTACCACATTACCACATTACCACTTGTTAATGTGTGTTA
CTTTCATGAGAAATGCTAAAATATAAAAGCCAGAGTGTGGCAGCACTCTAGCTTTTAAAAAAGAAACTACCCTTTAATAG
GAGGGTCCTCGTGTAGACACTTTTCCTTTACAGTAATGTAAATAGGATGAGTGTCTTTTTTTC

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5