Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1884211..1884713 Replicon chromosome
Accession NZ_CP080255
Organism Escherichia coli strain SJC33

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag KZ313_RS08925 Protein ID WP_000767820.1
Coordinates 1884459..1884713 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KZ313_RS08920 Protein ID WP_001259255.1
Coordinates 1884211..1884462 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZ313_RS08895 (1879389) 1879389..1880456 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
KZ313_RS08900 (1880456) 1880456..1881526 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
KZ313_RS08905 (1881523) 1881523..1882827 - 1305 WP_001578572.1 histidinol dehydrogenase -
KZ313_RS08910 (1882833) 1882833..1883732 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
KZ313_RS08915 (1883878) 1883878..1883928 - 51 WP_001364200.1 his operon leader peptide -
KZ313_RS08920 (1884211) 1884211..1884462 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KZ313_RS08925 (1884459) 1884459..1884713 + 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KZ313_RS08930 (1884796) 1884796..1885620 + 825 WP_000754756.1 SDR family oxidoreductase -
KZ313_RS08935 (1885666) 1885666..1886595 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
KZ313_RS08940 (1886810) 1886810..1886872 + 63 WP_010723108.1 membrane protein YoeI -
KZ313_RS08945 (1886862) 1886862..1888220 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
KZ313_RS08950 (1888399) 1888399..1889457 + 1059 WP_033547227.1 thiosulfate utilization transporter TsuA/YeeE -
KZ313_RS08955 (1889471) 1889471..1889698 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T211480 WP_000767820.1 NZ_CP080255:1884459-1884713 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T211480 NZ_CP080255:1884459-1884713 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT211480 WP_001259255.1 NZ_CP080255:1884211-1884462 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT211480 NZ_CP080255:1884211-1884462 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References