Detailed information of TA system TA021148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE(toxin)
• Location: 1597323..1597948
• Replicon: chromosome
• Accession: NC_006513
• Organism: Aromatoleum aromaticum EbN1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EB_RS07525
• Protein ID: WP_041645992.1
• Coordinates: 1597607..1597948 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EB_RS07520
• Protein ID: WP_011237352.1
• Coordinates: 1597323..1597610 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EB_RS07490	1592931..1593719	+	789	WP_011237347.1	IS21-like element ISAzo12 family helper ATPase IstB	-
EB_RS07495	1594190..1595386	-	1197	Protein_1485	DEAD/DEAH box helicase	-
EB_RS07500	1595383..1595961	-	579	WP_011237349.1	YdaU family protein	-
EB_RS24180	1595958..1596395	-	438	WP_041645987.1	hypothetical protein	-
EB_RS07510	1596395..1596589	-	195	WP_041645990.1	hypothetical protein	-
EB_RS07515	1596662..1597222	-	561	WP_011237351.1	hypothetical protein	-
EB_RS07520	1597323..1597610	-	288	WP_011237352.1	helix-turn-helix domain-containing protein	Antitoxin
EB_RS07525	1597607..1597948	-	342	WP_041645992.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EB_RS07530	1597977..1599338	-	1362	WP_011237354.1	integrase family protein	-
EB_RS07540	1599848..1600351	-	504	WP_011237355.1	hypothetical protein	-
EB_RS07545	1600649..1601149	-	501	WP_041645995.1	flavin reductase family protein	-
EB_RS07550	1601148..1602335	+	1188	WP_157866705.1	A/G-specific adenine glycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1581674..1599338	17664

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (36-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-79)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12790.72 Da
• Isoelectric Point: 10.1223

>T21148 WP_041645992.1 NC_006513:c1597948-1597607 [Aromatoleum aromaticum EbN1]
MQTVAELPEYIRTADKLLSAEERQDIIRYLAEHPKDGDLMEGTGGVRKLRWRRGGQGKSGGVRVIYYFHDDAMPLYLLTL
FAKGDRANLSKAERNELAGLTTLLVKAWRGKRA

Nucleotide

• Length: 342 bp

>T21148 NC_006513:c1597948-1597607 [Aromatoleum aromaticum EbN1]
ATGCAAACCGTTGCCGAGCTGCCCGAGTACATCCGCACCGCCGACAAGCTGCTGAGCGCCGAAGAGCGGCAAGACATCAT
CCGCTATCTGGCCGAGCACCCGAAGGACGGCGATCTAATGGAAGGCACGGGAGGCGTGCGCAAATTGCGCTGGCGGCGCG
GTGGGCAGGGCAAGAGCGGGGGCGTTCGCGTCATCTACTACTTCCACGACGACGCCATGCCGCTGTACCTGTTGACGCTG
TTCGCCAAGGGCGACCGGGCCAACCTGAGCAAGGCGGAGCGCAACGAACTGGCGGGATTGACGACGCTACTGGTCAAAGC
ATGGAGAGGGAAACGAGCATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10427.97 Da
• Isoelectric Point: 11.3253

>AT21148 WP_011237352.1 NC_006513:c1597610-1597323 [Aromatoleum aromaticum EbN1]
MSTAFDSIKRGLMEAIDHAEGRAPATRVHRPRPVDVKALRAKVGMTQEQFAARFGFSTATLRHWERGDRTPHGPALVLLN
VIDRNPAAVIEALSA

Nucleotide

• Length: 288 bp

>AT21148 NC_006513:c1597610-1597323 [Aromatoleum aromaticum EbN1]
ATGAGCACCGCATTCGACAGCATCAAGCGCGGTTTGATGGAGGCCATCGATCACGCCGAAGGCCGCGCCCCGGCAACCCG
CGTTCACCGCCCGCGCCCGGTGGACGTGAAGGCGTTACGCGCCAAGGTGGGCATGACGCAGGAGCAGTTCGCGGCCCGCT
TCGGATTCTCGACTGCAACCCTTCGTCATTGGGAGCGAGGCGATCGCACGCCACACGGCCCCGCACTCGTGCTGCTGAAC
GTCATCGATCGCAATCCGGCCGCTGTCATCGAGGCCCTGAGCGCCTGA