Detailed information of TA system TA211479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1124628..1125355
• Replicon: chromosome
• Accession: NZ_CP080255
• Organism: Escherichia coli strain SJC33

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: KZ313_RS05335
• Protein ID: WP_000547555.1
• Coordinates: 1124628..1124939 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KZ313_RS05340
• Protein ID: WP_000126294.1
• Coordinates: 1124936..1125355 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZ313_RS05310 (1120563)	1120563..1122272	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
KZ313_RS05315 (1122282)	1122282..1122824	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
KZ313_RS05320 (1122824)	1122824..1123591	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
KZ313_RS05325 (1123588)	1123588..1123998	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
KZ313_RS05330 (1123991)	1123991..1124461	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
KZ313_RS05335 (1124628)	1124628..1124939	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
KZ313_RS05340 (1124936)	1124936..1125355	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
KZ313_RS05345 (1125434)	1125434..1126858	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
KZ313_RS05350 (1126867)	1126867..1128324	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
KZ313_RS05355 (1128584)	1128584..1129594	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
KZ313_RS05360 (1129743)	1129743..1130270	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T211479 WP_000547555.1 NZ_CP080255:1124628-1124939 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T211479 NZ_CP104869:c5110248-5109901 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT211479 WP_000126294.1 NZ_CP080255:1124936-1125355 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT211479 NZ_CP104869:c5110509-5110258 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Toxin

• AlphaFold DB: V0YLE2